Timstof Flex Maldi-2

The post-ionization laser is built as an add-on to the timsTOF fleX. All functions are exactly the same on this new machine and no physical hardware changes are needed to activate post-ionization. By just one click in the software the user will be able to change between post-ionization and traditional MALDI mode. This is done in seconds and allows for direct comparison of the two different ionization processes. You do not have to be a professional MALDI user to use MALDI-2 as it is easy to handle and therefore also suitable for beginners.

涉及从组织切片解吸的小分子（例如脂质组学and代谢组学typically involve biomolecules from a wide variety of chemical classes. These studies benefit from MALDI-2 as the technique provides access to many more compounds, providing unique insight into nature’s complexity. The combination of TIMS and MALDI-2 is uniquely powerful as richer ion yields from the post-ionization process produce mass spectra with higher information content. TIMS provides fast orthogonal separation that efficiently unravels these complex spectra where each m/z can contain many overlapping features. The result is not only the ability to extract information from single isobars, but also exact masses that have different ion mobilities. Collisional Cross Section (CCS) values are recorded for each one of the spectral components for comparison to databases or LC/MS results.

A major limiting factor in modern drug development is use of the “well-stirred model” which homogenizes organs and tissues prior to analysis and quantitation with LC-MS studies. This approach is well-suited to providing exact amounts of drugs and metabolites within a target organ, but not readily compatible with pathology methods that seek to describe physiological effects of drug compounds.
MALDI Imaging has made a significant impact towards the conversion of plasma

通过查明组织中药物和代谢产物的确切位置来进行组织模型。MALDI-2通过提高总体敏感性，从而对更广泛的给药水平进行定量，从而增强了药物研究的分子成像。此外，MALDI-2观察到的分子类种类增加，扩展了成像对涉及异种生物和内源分子的更多药物项目的适用性。