timsTOF fleX Bibliography
Introduction
timsTOF fleX Bibliography
The timsTOF fleX is a high performance UHR-OTOF system with integrated dual ESI/MALDI source, exhibiting high speed and robust MALDI Imaging for all imaginable X-Omics analyses. The additional trapped ion mobility feature adds another separation dimension to unravel complex data and make annotations in MALDI Images more reliable through CCS-matching. Since ASMS 2020 we switched to the next level to gain higher sensitivity for a broad range of compounds and equipped the instrument with a second laser allowing for postionization (MALDI-2).
MALDI-2 uses laser based postionization to enhance and enrich the MALDI experiment, providing access to chemical classes typically opaque to MALDI, at unprecedented sensitivity (2-3 orders of magnitude compared to traditional MALDI) Postionization significantly boosts ion yields for many different analytes and reduces the ever-challenging ion suppression effects in MALDI imaging.
The peer-reviewed and in-peer-reviewing communications listed below is a non-exhaustive list from the work initiated on the first timsTOF fleX (MALDI-2) instruments in the field in the years 2019-2021 and many more are to come.
timsTOF fleX
timsTOF fleX
| Title | Author | Publication | Link | year | Application |
|---|---|---|---|---|---|
| Detection of small molecule concentration gradients in ocular tissues and humours | Boughton, B.A.; Thomas, O. R. B.; Demarais, N. J. et al. | Journal of Mass Spectrometry 55(4), 2019, e4460 | https://doi.org/10.1002/jms.4460 | 2019 | Biology |
| Hydroperoxylated vs Dihydroxylated Lipids: Differentiation of Isomeric Cardiolipin Oxidation Products by Multidimensional Separation Techniques | Helmer, P.O.; Behrens, A.; Rudt, E. et al. | Analytical Chemistry 92(17), 2020, 12010-12016 | https://doi.org/10.1021/acs.analchem.0c02605 | 2020 | Biology |
| In situ isobaric lipid mapping by MALDI–ion mobility separation–mass spectrometry imaging | Fu, T.; Oetjen, J.; Chapelle, M. et al. | Journal of Mass Spectrometry 55(9), 2020, e4531 | https://doi.org/10.1002/jms.4531 | 2020 | Biology |
| Resolving the Complexity of Spatial Lipidomics Using MALDI TIMS Imaging Mass Spectrometry | Djambazova, K. V.; Klein, D. R.; Migas, L. G. et al. | Analytical Chemistry 92(19), 2020, 13290-13297 | https://doi.org/10.1021/acs.analchem.0c02520 | 2020 | Biology |
| Spatial Metabolomics of the Human Kidney using MALDI Trapped Ion Mobility Imaging Mass Spectrometry. | Neumann, E. K.;Migas, L. G.; Allen, J. L. et al. | Analytical Chemistry 92(19), 2020, 13084-13091 | https://doi.org/10.1021/acs.analchem.0c02051 | 2020 | Biology |
| Multiplexed imaging mass spectrometry of the extracellular matrix using serial enzyme digests from formalin-fixed paraffin-embedded tissue sections | Clift, C. L.; Drake, R. R.; Mehta, A. et al. | Analytical and Bioanalytical Chemistry 413, 2021, 2709-2719 | https://doi.org/10.1007/s00216-020-03047-z | 2021 | Biology |
| Preserved and variable spatial-chemical changes of lipids across tomato leaves in response to central vein wounding reveals potential origin of linolenic acid in signal transduction cascade. | Veličković, D.; Chu, R. K.; Henkel. C. et al. | Journal of Plant-Environment Interactions 2(1), 2021, 28-35 | https://doi.org/10.1002/pei3.10038 | 2021 | Biology |
| Complete spatial characterisation of N-glycosylation upon striatal neuroinflammation in the rodent brain. | Rebelo, A. L.; Gubinelli, F.; Roost, P. et al. | Journal of Neuroinflammation 18, 2021, Article number: 116 | https://doi.org/10.1186/s12974-021-02163-6 | 2021 | Biology |
| Morphometric Cell Classification for Single-Cell MALDI-Mass Spectrometry Imaging | Ščupáková,, K.; Dewez, F.; Walch, A. K. et al. | Angewandte Chemie International Edition 59(40), 2020, 17447-17450 | https://doi.org/10.1002/anie.202007315 | 2020 | Biology - Clinical |
| Modulating Isoprenoid Biosynthesis Increases Lipooligosaccharides and Restores Acinetobacter baumannii Resistance to Host and Antibiotic Stress | Palmer, L. D.; Minor, K. E.; Mettlach, J. A. et al. | Cell Reports 32(10), 2020, 108129 | https://doi.org/10.1016/j.celrep.2020.108129 | 2020 | Biology - Clinical |
| Lipidomic profiling of clinical prostate cancer reveals targetable alterations in membrane lipid composition | Butler, L. M.; Mah, C. Y.; Machiels, J. et al. | Cancer Research, 2020, 3863 | https://doi.org/10.1158/0008-5472.CAN-20-3863 | 2020 | Biology - Clinical |
| Imaging Mass Spectrometry and Lectin Analysis of N-linked Glycans in Carbohydrate Antigen Defined Pancreatic Cancer Tissues | McDowell, C. T.; Klamer, Z.; Hall, J. et al. | Molecular & Cellular Proteomics 20, 2021, 100012 | https://doi.org/10.1074/mcp.RA120.002256 | 2021 | Biology - Clinical |
| Spatial differentiation of metabolism in prostate cancer tissue by MALDI-TOF MSI | Andersen, M. K.; Høiem, T. S.; Claes, B. S. R. et al. | Cancer & Metabolism 9, 2021, Article number: 9 | https://doi.org/10.1186/s40170-021-00242-z | 2021 | Biology - Clinical |
| β-赛克lodextrin-poly (β-Amino Ester) Nanoparticles Are a Generalizable Strategy for High Loading and Sustained Release of HDAC Inhibitors | Chaudhuri, S.; Fowler, M. J.; Baker, C. et al. | Applied Materials & Interface 13, 2021, 20960-20973 | https://doi.org/10.1021/acsami.0c22587 | 2021 | Biology - Clinical |
| Auto-aggressive CXCR6+ CD8 T cells cause liver immune pathology in NASH | Dudek, M.; Pfister, D.; Donakonda, S. et al. | Nature 592, 2021, 444-449 | https://doi.org/10.1038/s41586-021-03233-8 | 2021 | Biology - Clinical |
| CRL4AMBRA1 is a master regulator of D-type cyclins. | Simoneschi, D.; Rona, G.; Zhou, N. et al. | Nature 592, 2021, 789-793 | https://doi.org/10.1038/s41586-021-03445-y | 2021 | Biology - Clinical |
| Absolute Quantification of 2‐Hydroxyglutarate on Tissue by MALDI MSI for Rapid and Precise Identification of IDH Mutations in Human Glioma | Lan, C.; Li, H.; Wang, L. et al. | International Journal of Cancer, 2021 | https://doi.org/10.1002/ijc.33729 | 2021 | Biology - Clinical |
| High-Performance Molecular Imaging with MALDI Trapped Ion-Mobility Time-of-Flight (timsTOF) Mass Spectrometry | Spraggins, J. M.; Djambazova, K. V.; Rivera, E. S. et al. | Analytical Chemistry 91(22), 2019, 14552-14560 | https://doi.org/10.1021/acs.analchem.9b03612 | 2019 | Chemistry – Basic Research |
| Automated Chiral Analysis of Amino Acids Based on Chiral Derivatization and Trapped Ion Mobility–Mass Spectrometry | Will, J. M.; Behrens, A.; Macke, M. | Analytical Chemistry 93(2), 2021, 878-885 | https://doi.org/10.1021/acs.analchem.0c03481 | 2021 | Chemistry – Basic Research |
| Automated Biomarker Candidate Discovery in Imaging Mass Spectrometry Data Through Spatially Localized Shapley Additive Explanations | Tideman, L. E. M.; Migas, L. G.; Djambazova, K. V. et al. | Analytica Chimica Acta 1177, 2021, 338522 | https://doi.org/10.1016/j.aca.2021.338522 | 2021 | Chemistry – Basic Research |
| MS Imaging‐Guided Microproteomics for Spatial Omics on a Single Instrument | Dewez, F.; Oetjen, J.; Henkel, C. et al. | Proteomics 20, 2020, 1900369 | https://doi.org/10.1002/pmic.201900369 | 2020 | Chemistry – Basic Research |
| Mass‐Spectrometric Imaging of Electrode Surfaces—a View on Electrochemical Side Reactions | Fangmeyer, J.; Behrens, A.; Gleede, B. et al. | Angewandte Chemie 132(46), 2020, 20608-20613 | https://doi.org/10.1002/ange.202010134 | 2020 | Chemistry – Basic Research |
| A mass spectrometry-based approach gives new insight into organotin–protein interactions | Will, J. M.; Erbacher, C.; Sperling, M. et al. | Metallomics 12(11), 2020, 1702-1712 | https://doi.org/10.1039/d0mt00171f | 2020 | Chemistry – Basic Research |
| Rapid N-Glycan Profiling of Serum and Plasma by a Novel Slide-Based Imaging Mass Spectrometry Workflow | Blaschke, C. R. K.; Black, A. P.; Mehta, A. S. et al. | Journal of the American Society for Mass Spectrometry 31, 2020, 2511-2520 | https://doi.org/10.1021/jasms.0c00213 | 2020 | Chemistry – Basic Research |
| Simulation of the oxidative metabolization pattern of netupitant, a NK1 receptorantagonist, by electrochemistry coupled to mass spectrometry | Chira, R.; Fangmeyer, J.; Neaga, I. O. et al. | Journal of Pharmaceutical Analysis - in press, 2021 | https://doi.org/10.1016/j.jpha.2021.03.011 | 2021 | Chemistry – Basic Research |
| Removal of optimal cutting temperature (O.C.T.) compound from embedded tissue for MALDI imaging of lipids | Truong, J. X. M.; Spotbeen, X.; White, J. et al. | Analytical and Bioanalytical Chemistry 413, 2021, 2695-2708 | https://doi.org/10.1007/s00216-020-03128-z | 2021 | Chemistry – Basic Research |
| A TIMS-TOF mass spectrometry study of disaccharides from in-situ derivatization ESI with 3-pyridinylboronate | Lia, L.; Yua, J.; Xiea, C. et al. | Analyst 146, 2021, 75 | https://doi.org/10.1039/D0AN01677B | 2021 | Chemistry – Basic Research |
| Complementing Matrix-Assisted Laser Desorption Ionization-Mass Spectrometry Imaging with Chromatography Data for Improved Assignment of Isobaric and Isomeric Phospholipids Utilizing Trapped Ion Mobility-Mass Spectrometry | Helmer, P. O.; Nordhorn, I. D.; Korf, A. et al. | Analytical Chemistry 93, 2021, 2135-2143 | https://doi.org/10.1021/acs.analchem.0c03942 | 2021 | Chemistry – Basic Research |
| Aliquoting of isobaric labeling reagents for low concentration and single cell proteomics samples | Yuan, Y.; Orsburn, B. C. | bioRxiv pre-print, 2021 | https://doi.org/10.1101/2021.06.23.449560 | 2021 | Chemistry – Basic Research |
| 检测和映射的血红蛋白变体在blood fingermarks by MALDI MS for suspect “profiling”. | Heaton, C.; Witt, M.; Cole, L. et al. | Analyst 146, 2021, 4290 | https://doi.org/10.1039/d1an00578b | 2021 | Chemistry – Basic Research |
| OpenTIMS, TimsPy, and TimsR: Open and Easy Access to timsTOF Raw Data | Łącki, M. K.; Startek, M. P.; Brehmer, S. et al. | Journal of Proteome Research 20, 2021, 2122-2129 | https://doi.org/10.1021/acs.jproteome.0c00962 | 2021 | Chemistry – Basic Research |
| Rapid visualization of lipopeptides and potential bioactive groups of compounds by combining ion mobility and MALDI imaging mass spectrometry | McCann, A.; Kune, C.; La Rocca, R. et al. | Drug Discovery Today: Technologies - in press, 2021 | https://doi.org/10.1016/j.ddtec.2021.08.003 | 2021 | Chemistry – Basic Research |
| Protocol for multimodal analysis of human kidney tissue by imaging mass spectrometry and CODEX multiplexed immunofluorescence | Neumann, E. K.; Patterson, N. H.; Allen, J. L. et al. | STAR Protocols 2(3), 2021 100747 | https://www.sciencedirect.com/science/article/pii/S2666166721004548 | 2021 | Protocol |
| High Spatial Resolution MALDI Imaging Mass Spectrometry of Fresh-Frozen Bone | Good, C. J.; Neumann, E. K.; Butrico, C. E. et al. | bioRxiv pre-print, 2021 | https://www.biorxiv.org/content/10.1101/2021.10.01.462831v2 | 2021 | Biology |
| Development of an electrochemical flow‐through cell for the fast and easy generation of isotopically labeled metabolite standards | Göldner, V.; Fangmeyer, J.; Karst, U. | Drug Testing and Analysis, 2021 | https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/dta.3175 | 2021 | Chemistry – Basic Research |
| Unraveling metabolic alterations in transgenic mouse model of Alzheimer's disease using MALDI MS imaging with 4-aminocinnoline-3-carboxamide matrix | Chen, Y.; Hu, D.; Zhao, L.; Tang, W.; Li, B. | Analytica Chimica Acta, 2021, 339337 pre-print | https://www.sciencedirect.com/science/article/abs/pii/S0003267021011636 | 2021 | Biology - Clinical |
| Oxygen‐Doped PAH Electrochromes: Difurano, Dipyrano, and Furano‐Pyrano Containing Naphthalene‐Cored Molecules | Fletcher-Charles, J.; Ferreira, R.R.; Abraham, M. et al. | European Journal of Organic Chemistry, 2021 | https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/ejoc.202101166 | 2021 | Chemistry – Basic Research |
| Evaluation of Therapeutic Collagen-Based Biomaterials in the Infarcted Mouse Heart by Extracellular Matrix Targeted MALDI Imaging Mass Spectrometry | Clift, C. L.; McLaughlin, S.; Muñoz, M. et al. | Journal of The American Society for Mass Spectrometry 32 (12), 2021, 2746-2754 | https://pubs.acs.org/doi/10.1021/jasms.1c00189 | 2021 | Biology - Clinical |
| Poly(2-vinylpyridine) as a reference compound for mass calibration in positive-ion matrix-assisted laser desorption/ionization-mass spectrometry on different instrumental platforms | Gross, J. H. | European Journal of Mass Spectrometry 27(5), 2021, 191-204 | https://journals.sagepub.com/doi/10.1177/14690667211055701 | 2021 | Chemistry – Basic Research |
| Direct N-Glycosylation Profiling of Urine and Prostatic Fluid Glycoproteins and Extracellular Vesicles | Blaschke, C. R. K.; Hartig, J. P.; Grimsley, G. et al. | Frontiers in Chemistry 9, 2021, 734280 | https://www.frontiersin.org/articles/10.3389/fchem.2021.734280/full | 2021 | Biology |
| Mass Spectrometry Imaging of Low-Molecular-Weight Phenols Liberated from Plastics | Xu, Q.; Tian, R.; Lu, C. et al. | Analytical Chemistry 93(40), 2021, 13703-13710 | https://pubs.acs.org/doi/10.1021/acs.analchem.1c03397 | 2021 | Chemistry – Basic Research |
| Microfluidic Electrochemistry Meets Trapped Ion Mobility Spectrometry and High-Resolution Mass Spectrometry—In Situ Generation, Separation, and Detection of Isomeric Conjugates of Paracetamol and Ethoxyquin | Korzhenko, O.; Führer, P.; Göldner, V. et al. | Analytical Chemistry 93(37), 2021, 12740-12747 | https://pubs.acs.org/doi/10.1021/acs.analchem.1c02791 | 2021 | Chemistry – Basic Research |
| Traumatic brain injury induces region-specific glutamate metabolism changes as measured by multiple mass spectrometry methods | 苗圃, J. L.; Sowers, M. L.; Shavkunov, A. S. et al. | iScience 24(10), 2021, 103108 | https://www.cell.com/iscience/fulltext/S2589-0042(21)01076-2 | 2021 | Biology - Clinical |
| Retro Diels–Alder Fragmentation of Fulvene–Maleimide Bioconjugates for Mass Spectrometric Detection of Biomolecules | Stevens, K. G.; McFarlene, L. O.; Platts, K. et al. | Analytical Chemistry 93(36), 2021, 12204-12212 | https://pubs.acs.org/doi/10.1021/acs.analchem.1c00193 | 2021 | Chemistry – Basic Research |
timsTOF fleX MALDI-2
timsTOF fleX MALDI-2
| Title | Author | Publication | Link | year | Application |
|---|---|---|---|---|---|
| Molecular insights into symbiosis — mapping sterols in a marine flatworm-algae-system using high spatial resolution MALDI-2-MS imaging with ion mobility separation | Bien, T; Hambleton, E. A; Dreisewerd, K. et al. | Analytical and Bioanalytical Chemistry 413, 2021, 2767-2777 | https://doi.org/10.1007/s00216-020-03070-0 | 2021 | Biology |
| Spatial Distribution of Isobaric Androgens in Target Tissues Using Chemical Derivatization and MALDI-2 on a Trapped Ion Mobility Quadrupole Time-of-Flight Instrument | Mackay, C. L.; Soltwisch, J.; Heijs, B.; Smith, K. W.; Cruickshank, F. L.; Nyhuis, A.; Dreisewerd, K.; Cobice, D. | RSC Advances, 54, 2021 in-print | https://doi.org/10.1039/d1ra06086d | 2021 | Biology - Clinical |
| MALDI-2 on a Trapped Ion Mobility Quadrupole Time-of-Flight Instrument for Rapid Mass Spectrometry Imaging and Ion Mobility Separation of Complex Lipid Profiles | Soltwisch, J.; Heijs, B.; Koch, A. et. al | Analytical Chemistry 92, 2020, 8697-8703 | https://doi.org/10.1021/acs.analchem.0c01747 | 2020 | Chemistry – Basic Research |
| MALDI‑2 for the Enhanced Analysis of N‑Linked Glycans by Mass Spectrometry Imaging | Heijs, B.; Potthoff, A.; Soltwisch, J. et al. | Analytical Chemistry 92, 2020, 13904-13911 | https://doi.org/10.1021/acs.analchem.0c02732 | 2020 | Chemistry – Basic Research |
For Research Use Only. Not for use in clinical diagnostic procedures.