TimstofPro

捕获的离子迁移率光谱法（TIMS）是气相中首先是一种分离技术，除了HPLC和质谱法之外，它还以分离的添加尺寸解决样品复杂性，从而增加了峰值容量和对复合表征的信心。同样重要的是，TIMS设备还用于累积和浓缩质量和迁移率的浓缩离子，从而使灵敏度和速度以及分离的附加维度具有独特的提高。

Achieve near 100% duty cycle for shotgun proteomics
A near 100% duty cycle for high sensitivity, high speed shotgun proteomics can now be achieved with the dual TIMS technology. The novel design allows for ions to be accumulated in the front section, while ions in the rear section are sequentially released depending on their ion mobility. This process is called Parallel Accumulation Serial Fragmentation, or PASEF^®.

第二代双TIMS分析仪针对shot弹枪蛋白质组学需求进行了优化。由于其独特的几何离子，释放了从TIMS分析仪的第二部分中的移动性释放，而进一步的离子可以在TIMS分析仪的第一部分并行积累。使用平行的积累技术，获得接近100％的占空比，导致几乎没有离子损失。

In the timsTOF Pro an advanced segmented quadrupole mass filter is used for high ion transmission and isolation efficiency. The quadrupole mass position is synchronized with the elution times of the specific ions from the TIMS analyzer. Due to its ultra-high mass position switching time (< 1 ms) it enables the best performance for the PASEF^®method.

The only PASEF^®-enabled mass spectrometer
布鲁克（Bruker）在2016年推出了TIMS（被困的离子移动光谱法）技术，作为一种革命性的离子移动技术，在极其紧凑的设备中实现了前所未有的离子迁移率分辨率。第二代双TIMS分析仪现在启用PASEF^®(Parallel Accumulation Serial Fragmentation) that is setting new standards of uncompromised speed, sensitivity and resolution for shotgun proteomics.

Superior robustness, no cleaning required
许多女士struments used for shotgun proteomics require biweekly or monthly cleaning when run 24 hours a day on large sample cohorts, and performance degradation is noticeable over even shorter time periods. The superior robustness of the timsTOF Pro means that the instrument can be run 24/7 over many days or weeks without noticeable loss of sensitivity or other performance metrics.

The timsTOF Pro allows the analysis of hundreds of samples with minimal sample load (<200 ng) at sequencing speeds exceeding 100 Hz. All of this with uncompromised proteomic depth. This has changed the way proteomics is done, but it also unleashes a new constraint - data analysis. Modern researchers must process hundreds of samples of information dense content with the timsTOF Pro. For most pipelines this creates a bottleneck at the data analysis step. Bruker is innovating this step by introducing real-time database search capabilities called PaSER (Parallel database Search Engine in Real-time) which removes the data analysis hurdle. Using PaSER, as soon as a LC-MS run is completed, results are in hand or Run & Done.

MaxQuant/Perseus and PEAKS Studio data processing
An open-file data format allows researchers to work directly with the raw data and use industry leading software. MaxQuant has been adapted to manage 4-dimensional (4D) features in the space spanned by retention time, ion mobility,

mass, and signal intensity which benefit the identification and quantification of peptides, proteins, and posttranslational modifications. PEAKS Studio combines de novo sequencing with traditional database searches and is optimized for processing timsTOF raw data.