表面等离子体共振(SPR)

Sierra SPR-24 Pro

Industry-leading SPR system offering the highest performance from assay development to initial screening

High throughput

Array of eight channels/three spot enables 8800 interactions in 24 hours

sierra-spr-24-pro

Основные моменты

表面等离子体共振(SPR)

针对高核应用程序量身定制

Flexibility
Choose any combination from 1 to 8 needles for optimum performance, flexibility and range of applications.
强大的
Designed to perform all SPR applications and capable of handling crude samples such as cell lysates.
24传感器位置
同时分析多达8个样品在24个传感器点上注入。
Productivity
8x3通道/斑点阵列允许单个传感器芯片上的多个控件或目标,以最大程度地读取单个样品注入。

Особенности

Sierra SPR-24 Pro

Analytical biosensor for real-time, label-free characterization of molecular interactions

连续流动SPR分析
SPR-24 PRO系统是连续的流动生物传感器,将样品和试剂作为连续流动的流提供给检测传感器。当样品或试剂注射停止时,传感器将传递“连续流缓冲区”。

The formulation of the continuous flow buffer in SPR assays can have a significant impact on the molecular interactions being measured. In many applications, measuring target interactions in a variety of buffer environments is required.

Sierra SPR-24 Pro

Frame Inject

Sierra Spr-24 Pro系统中的框架注入特征允许研究依赖性结合 /作用模式研究。该功能仅需要每种缓冲条件的一小部分,该条件在使用时替换系统缓冲区。应研究的情况下发生的预交换以及解离。解离端后,更换帧溶液缓冲液,系统缓冲区再次流过微流体系统。因此,实验时间和试剂成本保持较低。可以将相同的缓冲液定向到所有流动池,也可以同时测定多达八个不同的缓冲液。

框架注射允许并行研究多达八个缓冲条件。
Sierra SPR-24 Pro

通过单个针头控制最大化性能

单个针头控制
Sierra Spr-24 Pro系统中的单个针控(INC)功能允许独立操作八个样品拾音针头。研究人员可以灵活地选择1-8针的任何组合来设计其最佳测定法。

Individual needle control maximizes the performance, flexibility, and scope of applications with the Sierra SPR-24 Pro, while also saving time, materials, and resources when throughput is not the main requirement.

八个针头探针头可以同时处理8个样品或试剂溶液。使用Inc功能,用户可以在分析过程中随时选择使用针的任何数字和/或组合。

Преимущества

Sierra SPR-24 Pro

按需吞吐量

Sierra Spr-24 Pro可以在各种应用程序中提供更快的吞吐量,从每个传感器的动力学测定到使用多个在线控件的详细分析。

该系统使用Bruker的流体动力隔离(HI)连续流微流体技术来解决流动流到24个SPRS的样品+传感器阵列。当结合an optional plate handling robot, the Sierra SPR-24 Pro instrument can assay more than 4400 samples per day, generating more than 8800 control subtracted binding responses.

Sierra Spr-24 Pro可以轻松地通过API集成到自动调度软件中。

Sierra SPR-24 Pro

吞吐性能

The flexible Sierra SPR-24 Pro system is ideal for high throughput applications. It offers 24 detection spots, which are addressed by 8 injection needles. Individual needle control (INC) gives researchers the option to choose any combination of 1 to 8 needles, to optimize the performance, flexibility and scope of applications.

The 24-sensor detection array is laid out in 8 flow cells, each containing 3 sensors. Up to 8 samples can be analyzed simultaneously, generating up to 24 binding measurements per analysis cycle. All 24 sensors can also be addressed fully independent of the others, providing maximum assay design flexibility.

The versatile system can also save time and materials in the lab, particularly if throughput is not the main requirement.

Up to eight buffers can be used simultaneously in the instrument, using the frame inject feature. The ability to evaluate different pHs, ionic strength, detergent or solvent concentration, as well as the effect of co-factors or inhibitors, in a single assay results in simpler, optimized assays that ultimately increase throughput and productivity. The four-sensor per channel design provides maximum flexibility for control analysis, while maintaining throughput for the most complex analyses.

Sierra SPR-24 Pro

Enhanced productivity

The design of Sierra SPR-24 Pro maximizes control analysis flexibility, enabling high throughput for the most complex analyses. Samples can be simultaneously assayed on two controls as well as on the active sensor.

Early analysis of non-specific binding to matrix proteins such as HSA or BSA can be critical. Additional in-line control not only halves the number of assay cycles required but can also improve data quality by eliminating potential variations between multiple sample preparations.

从初始测定开发到预定义的方法模板,Sierra SPR控制软件为每种用例提供​​了完美的解决方案。它将硬件的灵活性与易于使用的拖放方法编辑器结合在一起。定义明确的板和方法库支持个人工作流程结构,数据视图允许对流动池进行实时视觉监视。

Расходные материалы

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Sierra SPR-24 PRO

Applications of Surface Plasmon Resonance


Investigation of condition-dependent binding using frame inject

对条件依赖性结合的传统研究需要高的试剂成本以及冗长的运行时间。框架注入以低样本消耗以节省成本和时间。


How to influence ligand density using amine coupling

Finding the ideal immobilization level to obtain optimal kinetic measurements is critical. Here we examine the influencing parameters on different example targets.


筛选和表征小分子与蛋白质靶标的结合

Real-time, label-free (RT-LF) analysis by SPR detection is a powerful tool for the biophysical characterization of small molecule drugs and drug candidates.


Screening and characterization of biotherapeutics

Real-time, label-free (RT-LF) analysis by SPR detection is a powerful tool for the biophysical characterization of protein therapeutics.