表面等离子体共振(SPR)

Sierra SPR-24 Pro

Industry-leading SPR system offering the highest performance from assay development to initial screening

High throughput

Array of eight channels/three spot enables 8800 interactions in 24 hours

sierra-spr-24-pro

distaques.

表面等离子体共振(SPR)

为高内容应用程序量身定制

Flexibility
Choose any combination from 1 to 8 needles for optimum performance, flexibility and range of applications.
强壮的
Designed to perform all SPR applications and capable of handling crude samples such as cell lysates.
24传感器位置
同时分析高达8个样品注入24个传感器斑点。
Productivity
8x3通道/点阵列允许在单个传感器芯片上进行多个控制或目标,以便每单个样品注入最大限度地读出。

características.

Sierra SPR-24 Pro

Analytical biosensor for real-time, label-free characterization of molecular interactions

连续流程分析
SPR-24 Pro系统是连续流体体传感器,将样品和试剂输送到检测传感器,作为连续流动的流。当样品或试剂注入停止时,通过传感器通过“连续流动缓冲器”。

The formulation of the continuous flow buffer in SPR assays can have a significant impact on the molecular interactions being measured. In many applications, measuring target interactions in a variety of buffer environments is required.

Sierra SPR-24 Pro

Frame Inject

Sierra Spr-24 Pro系统中的帧注射功能允许调查条件相关的结合/动作研究模式。该功能只需要在使用时替换系统缓冲区的每个缓冲区条件的一小部分。预关联,以及在应研究的条件下发生解离。在解离结束后,替换框溶液缓冲液,并且系统缓冲液再次流过微流体系统。因此,实验时间和试剂成本保持低。可以将相同的缓冲液引导到所有流动细胞,或者可以同时测定最多八种不同的缓冲液。

帧注射允许调查最多八个缓冲条件并行。
Sierra SPR-24 Pro

通过单个针控制最大化性能

单个针控制
The individual needle control (INC) feature in the Sierra SPR-24 Pro system allows the eight sample pickup needles to be operated independently as well as in unison. Researchers have the flexibility to choose any combination of 1 – 8 needles to design their optimal assay.

Individual needle control maximizes the performance, flexibility, and scope of applications with the Sierra SPR-24 Pro, while also saving time, materials, and resources when throughput is not the main requirement.

八针头探针头可以同时处理8个样品或试剂溶液。使用INC功能,用户可以选择在分析期间的任何时间使用针头的任何数字和/或组合。

优点

Sierra SPR-24 Pro

吞吐量需求

Sierra Spr-24 Pro通过在每个传感器上的具有不同目标的动力测定中提供更快的吞吐量,以在每个传感器上具有不同的目标,以进行多种在线控制的详细分析。

该系统使用Bruker的流体动力学隔离(HI)连续流体微流体技术来解决样品,因为流动的流在24点SPR上+传感器阵列。当结合an optional plate handling robot, the Sierra SPR-24 Pro instrument can assay more than 4400 samples per day, generating more than 8800 control subtracted binding responses.

Sierra Spr-24 Pro可以通过API轻松集成到自动调度软件中。

Sierra SPR-24 Pro

吞吐量具有性能

The flexible Sierra SPR-24 Pro system is ideal for high throughput applications. It offers 24 detection spots, which are addressed by 8 injection needles. Individual needle control (INC) gives researchers the option to choose any combination of 1 to 8 needles, to optimize the performance, flexibility and scope of applications.

The 24-sensor detection array is laid out in 8 flow cells, each containing 3 sensors. Up to 8 samples can be analyzed simultaneously, generating up to 24 binding measurements per analysis cycle. All 24 sensors can also be addressed fully independent of the others, providing maximum assay design flexibility.

The versatile system can also save time and materials in the lab, particularly if throughput is not the main requirement.

Up to eight buffers can be used simultaneously in the instrument, using the frame inject feature. The ability to evaluate different pHs, ionic strength, detergent or solvent concentration, as well as the effect of co-factors or inhibitors, in a single assay results in simpler, optimized assays that ultimately increase throughput and productivity. The four-sensor per channel design provides maximum flexibility for control analysis, while maintaining throughput for the most complex analyses.

Sierra SPR-24 Pro

Enhanced productivity

The design of Sierra SPR-24 Pro maximizes control analysis flexibility, enabling high throughput for the most complex analyses. Samples can be simultaneously assayed on two controls as well as on the active sensor.

Early analysis of non-specific binding to matrix proteins such as HSA or BSA can be critical. Additional in-line control not only halves the number of assay cycles required but can also improve data quality by eliminating potential variations between multiple sample preparations.

从初始测定开发到预定义的方法模板,Sierra SPR控制软件为每个用例提供完美的解决方案。它将硬件的灵活性与易于使用的拖放方法编辑器相结合。明确定义的板和方法库支持个人工作流程结构,数据视图允许实时视觉监控流量。

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Mais informações

Sierra SPR-24 PRO

Applications of Surface Plasmon Resonance


Investigation of condition-dependent binding using frame inject

传统的依赖性结合调查需要高试剂成本以及冗长的运行时间。帧注入可在低样本消耗下提供成本和时间节省。


How to influence ligand density using amine coupling

Finding the ideal immobilization level to obtain optimal kinetic measurements is critical. Here we examine the influencing parameters on different example targets.


小分子与蛋白质靶标结合的筛选与表征

Real-time, label-free (RT-LF) analysis by SPR detection is a powerful tool for the biophysical characterization of small molecule drugs and drug candidates.


Screening and characterization of biotherapeutics

Real-time, label-free (RT-LF) analysis by SPR detection is a powerful tool for the biophysical characterization of protein therapeutics.