Multiphoton Microscopes
Leading advances in neuroscience with fast, deep and large field-of-view multiphoton imaging
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See Deeper into Brain Activity
多光子显微镜（MPM）是一种强大的荧光显微镜技术，可以在前所未有的深度尺度下生成生物结构和过程的3D图像。通过将两个光子与一半能量组合到标本中激发荧光团（合成和天然）的一半，MPM可以揭示出在活组织内部深处发生的动态生物学过程。MPM除了在前所未有的深度提供3D图像外，还可以减少光漂白，并通过激发波长扩展照明造成的损害。
布鲁克（Bruker）的Ultima Multiphoton成像技术在科学发现的最前沿，是数十年来与世界各地主要神经科学家的亲密合作和实验室经验的直接结果。这些专家的参与导致了仅在Ultima Systems上找到的许多独特功能和功能，例如我们的模块化设置，Incomporated PhotoStimulation Light Path和功能丰富的Prairie View Software。
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多光子显微镜的模块
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多光子显微镜网络研讨会系列
Hear from researchers and experts on best practices, new techniques and discoveries, and ideas for new applications using two-photon microscopy.
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Browse Multiphoton Microscopy Articles
- Basu J，Zaremba JD，Cheung SK，Hitti FL，Zemelman BV，Losonczy A，Siegelbaum SA。Gating of hippocampal activity, plasticity, and memory by entorhinal cortex long-range inhibition. (2016) Science, 351(6269):aaa5694. doi: 10.1126/science.aaa5694
- Marshel JH，Kim YS，Machado TA，Quirin S，Benson B，Kadmon J，Raja C，Chibukhchyan A，Ramakrishnan C，Inoue M，Inoue M，Shane JC，McKnight DJ，Yoshizawa S，Kato He，Ganguli S，Ganguli S，Deisseroth K.Cortical layer-specific critical dynamics triggering perception.(2019) Science, 365(6453):eaaw5202. doi: 10.1126/science.aaw5202
- Oh WC, Lutzu S, Castillo PE, Kwon HB.GABA在发育中的小鼠皮层中诱导的从头突触发生。(2016) Science, 353(6303):1037-1040. doi: 10.1126/science.aaf5206
- Packer AM，Russell LE，Dalgleish HW，HäusserM。Simultaneous all-optical manipulation and recording of neural circuit activity with cellular resolution in vivo.（2015）NAT方法，12（2）：140-6。doi：10.1038/nmeth.3217
- Rowan MJ，Delcanto G，Yu JJ，Kamasawa N，Christie JM。轴突中K（+）通道聚类对动作电位持续时间的突触级测定。(2016) Neuron, 91(2):370-83. doi: 10.1016/j.neuron.2016.05.035
- Zhang Z, Russell LE, Packer AM, Gauld OM, Häusser M.Closed-loop all-optical interrogation of neural circuits in vivo.(2018) Nat Methods, 15(12):1037-1040. doi: 10.1038/s41592-018-0183-z
- Hartmann DA, Berthiaume AA, Grant RI, Harrill SA, Koski T, Tieu T, McDowell KP, Faino AV, Kelly AL, Shih AY.Brain capillary pericytes exert a substantial but slow influence on blood flow.(2021) Nat Neurosci. doi: 10.1038/s41593-020-00793-2. Epub ahead of print. PMID: 33603231
- Li J，Ellis KE，Caron SJC。Photo-labeling neurons in the Drosophila brain.(2021) STAR Protoc., 2(1):100381. doi: 10.1016/j.xpro.2021.100381. PMID: 33733243; PMCID: PMC7941087
- Mann K, Deny S, Ganguli S, Clandinin TR.Coupling of activity, metabolism and behaviour across the Drosophila brain.（2021）自然。doi：10.1038/s41586-021-03497-0。Epub在印刷前。PMID：33911283
- McDowell KP, Berthiaume AA, Tieu T, Hartmann DA, Shih AY.VasoMetrics: unbiased spatiotemporal analysis of microvascular diameter in multi-photon imaging applications.(2021) Quant Imaging Med Surg., 11(3):969-982. doi: 10.21037/qims-20-920. PMID: 33654670; PMCID: PMC7829163
- Merlini M, Rafalski VA, Ma K, Kim KY, Bushong EA, Rios Coronado PE, Yan Z, Mendiola AS, Sozmen EG, Ryu JK, Haberl MG, Madany M, Sampson DN, Petersen MA, Bardehle S, Tognatta R, Dean T Jr, Acevedo RM, Cabriga B, Thomas R, Coughlin SR, Ellisman MH, Palop JJ, Akassoglou K.Microglial Gi-dependent dynamics regulate brain network hyperexcitability.(2021) Nat Neurosci.., 24(1):19-23. doi: 10.1038/s41593-020-00756-7. Epub 2020 Dec 14. PMID: 33318667
- Pandey P，Singh A，Kaur H，Ghosh-Roy A，Babu K.Increased dopaminergic neurotransmission results in ethanol dependent sedative behaviors in Caenorhabditis elegans.（2021）PLOS Genet。，17（2）：E1009346。doi：10.1371/journal.pgen.1009346。Epub在印刷前。PMID：33524034
- Simões JM, Levy JI, Zaharieva EE, Vinson LT, Zhao P, Alpert MH, Kath WL, Para A, Gallio M.Robustness and plasticity in Drosophila heat avoidance.(2021) Nat Commun., 12(1):2044. doi: 10.1038/s41467-021-22322-w. PMID: 33824330; PMCID: PMC8024383
- Sipe GO, Petravicz J, Rikhye RV, Garcia R, Mellios N, Sur M.Astrocyte glutamate uptake coordinates experience-dependent, eye-specific refinement in developing visual cortex.（2021）神经胶质。doi：10.1002/Glia.23987。Epub在印刷前。PMID：33675674
- Turner MH, Mann K, Clandinin TR.该连接组预测果蝇大脑之间的静息状态功能连通性。（2021）Curr Biol。，S0960-9822（21）00343-2。doi：10.1016/j.cub.2021.03.004。Epub在印刷前。PMID：33770490
- Walsh AJ, Mueller KP, Tweed K, Jones I, Walsh CM, Piscopo NJ, Niemi NM, Pagliarini DJ, Saha K, Skala MC.Classification of T-cell activation via autofluorescence lifetime imaging.(2021) Nat Biomed Eng., 5(1):77-88. doi: 10.1038/s41551-020-0592-z. Epub 2020 Jul 27. PMID: 32719514; PMCID: PMC7854821
- Allegra M, Posani L, Gómez-Ocádiz R, Schmidt-Hieber C.Differential Relation between Neuronal and Behavioral Discrimination during Hippocampal Memory Encoding.（2020）Neuron，108（6）：1103-1112.e6。doi：10.1016/j.neuron.2020.09.032。Epub 2020年10月16日。PMID：33068531;PMCID：PMC7772055。
- Bacmeister，C.M.，Barr，H.J.，McClain，C.R.，Thornton，M.A.，Nettles，D.，Welle，C.G。，＆Hughes，E。G.运动学习可以通过新的和幸存的少突胶质细胞来促进再髓。(2020) Nature Neuroscience, 23(7), 819–831. doi: 10.1038/s41593-020-0637-3
- Bojarskaite，L.，Bjørnstad，D.M.，Pettersen，K.H.，Cunen，C.，Hermansen，G.H.，Åbjørsbråtråten，K.S.Astrocytic Ca2+ signaling is reduced during sleep and is involved in the regulation of slow wave sleep.（2020）自然通讯，11（1）。doi：10.1038/s41467-020-17062-2-2
- Castello-Waldow TP，Weston G，Ulivi AF，Chenani A，Loewenstein Y，Chen A，Attardo A.具有稳定兴奋性连通性的海马神经元成为神经元表示的一部分。(2020) PLoS Biol., 18(11):e3000928. doi: 10.1371/journal.pbio.3000928. PMID: 33141818; PMCID: PMC7665705.
- Chen Q, Deister CA, Gao X, Guo B, Lynn-Jones T, Chen N, Wells MF, Liu R, Goard MJ, Dimidschstein J, Feng S, Shi Y, Liao W, Lu Z, Fishell G, Moore CI, Feng G.Dysfunction of cortical GABAergic neurons leads to sensory hyper-reactivity in a Shank3 mouse model of ASD.(2020) Nat >。, 23(4): 520 - 532。doi: 10.1038 /s41593-020-0598-6. Epub 2020 Mar 2. PMID: 32123378; PMCID: PMC7131894
- Dalgleish, H. W. P., Russell, L. E., Packer, A. M., Roth, A., Gauld, O. M., Greenstreet, F., … Häusser, M.有多少神经元足以感知皮质活性？（2020）Elife，9，1-99。doi：10.7554/elife.58889
- Damisah，E。C.，Hill，R.A.，Rai，A.，Chen，F.，Rothlin，C.V，Ghosh，S。，＆Grutzendler，J。Astrocytes and microglia play orchestrated roles and respect phagocytic territories during neuronal corpse removal in vivo.(2020) Science Advances. 6. eaba3239. doi: 10.1126/sciadv.aba3239
- Durand-de Cuttoli R, Chauhan PS, Pétriz Reyes A, Faure P, Mourot A, Ellis-Davies GCR.Optofluidic control of rodent learning using cloaked caged glutamate.(2020) Proc Natl Acad Sci U S A,117(12):6831-6835. doi: 10.1073/pnas.1920869117. Epub 2020 Mar 9. PMID: 32152102; PMCID: PMC7104027
- Eschbach，C.，Fushiki，A.，Winding，M.，Schneider-Mizell，C.M.，Shao，M.，Arruda，R.，…Zlatic，M。Recurrent architecture for adaptive regulation of learning in the insect brain.(2020) Nature Neuroscience, 23(4), 544–555. doi: 10.1038/s41593-020-0607-9
- Evans RC, Twedell EL, Zhu M, Ascencio J, Zhang R, Khaliq ZM.Functional Dissection of Basal Ganglia Inhibitory Inputs onto Substantia Nigra Dopaminergic Neurons.(2020) Cell Rep., 32(11):108156. doi: 10.1016/j.celrep.2020.108156.
- 乔治SS，斯蒂尔·CR，里奇·AJ。大鼠听觉内部毛细胞机械传导和立体膜膜扩散率类似地通过钙调节。（2020）Iscience，23（12）：101773。doi：10.1016/j.isci.2020.101773。PMID：33294782;PMCID：PMC7689183。
- Graves，S.M.，Xie，Z.，Stout，K.A.，Zampese，E.单胺氧化酶线粒体穿梭的多巴胺代谢激活了电子传输链。(2020) Nature Neuroscience, 23(1), 15–20. doi: 10.1038/s41593-019-0556-3
- 哈东J, Letzkus JJ.Inhibitory plasticity in layer 1 - dynamic gatekeeper of neocortical associations.(2020) Curr Opin Neurobiol., 67:26-33. doi: 10.1016/j.conb.2020.06.003. Epub ahead of print. PMID: 32818814.
- Heath, S. L., Christenson, M. P., Oriol, E., Saavedra-Weisenhaus, M., Kohn, J. R., & Behnia, R.果蝇光感受器中的色彩编码的电路机制。（2020）当前生物学，30（2），264-275.e8。doi：10.1016/j.cub.2019.11.075
- Huda, R., Sipe, G. O., Breton-Provencher, V., Cruz, K. G., Pho, G. N., Adam, E., … Sur, M.Distinct prefrontal top-down circuits differentially modulate sensorimotor behavior.（2020）自然通讯，11（1）。doi: 10.1038 /s41467-020-19772-z
- Ingiosi，A.M.，Hayworth，C.R.，Harvey，D.O.，Singletary，K.G.，Rempe，M.J.，Wisor，J.P。，＆Frank，M。G.A Role for Astroglial Calcium in Mammalian Sleep and Sleep Regulation.(2020) Current Biology, 30(22), 4373-4383.e7. doi: 10.1016/j.cub.2020.08.052
- Kaufman，A。M.，Geiller，T。，＆Losonczy，A。在空间奖励学习过程中，位于海马CA1中层层的作用将细胞重组。(2020) Neuron, 105(6), 1018-1026.e4. doi: 10.1016/j.neuron.2019.12.029
- Kim，T.，Chaloner，F.A.，Cooke，S.F.，Harnett，M.T。，＆Bear，M。F.Opposing Somatic and Dendritic Expression of Stimulus-Selective Response Plasticity in Mouse Primary Visual Cortex.(2020) Frontiers in Cellular Neuroscience, 13. doi: 10.3389/fncel.2019.00555
- Levy，S.，Lavzin，M.，Benisty，H.，Ghanayim，A.，Dubin，U.，Achvat，S.，…Schiller，J。一级运动皮层中的细胞型特异性结果表示。（2020）Neuron，107（5），954-971.E9。doi：10.1016/j.neuron.2020.06.006
- Li，J.，Mahoney，B.D.，Jacob，M.S。和Caron，S.J。C.Visual Input into the Drosophila melanogaster Mushroom Body.（2020）细胞报告，32（11）。doi：10.1016/j.celrep.2020.108138
- 刘C. J.，Roy，A.，Simons，A。A.，Farinella，D.M。和Kara，P。新皮层深层中合成染料的三光子成像。（2020）科学报告，10（1）。doi：10.1038/s41598-020-73438-w
- Morimoto, M. M., Nern, A., Zhao, A., Rogers, E. M., Wong, A., Isaacson, M. D., … Reiser, M. B.Spatial readout of visual looming in the central brain of Drosophila.（2020）Elife，9。doi：10.7554/elife.57685
- Pardi MB, Vogenstahl J, Dalmay T, Spanò T, Pu DL, Naumann LB, Kretschmer F, Sprekeler H, Letzkus JJ.A thalamocortical top-down circuit for associative memory.（2020）科学，370（6518）：844-848。doi：10.1126/science.ABC2399。PMID：33184213。
- Shani-Narkiss H, Vinograd A, Landau ID, Tasaka G, Yayon N, Terletsky S, Groysman M, Maor I, Sompolinsky H, Mizrahi A.Young adult-born neurons improve odor coding by mitral cells.(2020) Nat Commun., 11(1):5867. doi: 10.1038/s41467-020-19472-8. PMID: 33203831; PMCID: PMC7673122
- Sit KK, Goard MJ.小鼠视觉皮层中相干运动的分布和视网膜不对称处理。（2020）Nat Commun。，11（1）：3565。doi：10.1038/s41467-020-17283-5。PMID：32678087;PMCID：PMC7366664
- Tazerart，S.，Mitchell，D.E.，Miranda-Rottmann，S。和Araya，R。A spike-timing-dependent plasticity rule for dendritic spines.（2020）自然通讯，11（1）。doi：10.1038/s41467-020-17861-7
- Tong, L., Hill, R. A., Damisah, E. C., Murray, K. N., Yuan, P., Bordey, A., & Grutzendler, J.活大脑中血管壁细胞的成像和光遗传学调节。(2020) Nature Protocols. doi: 10.1038/s41596-020-00425-w
- Wang PY，Boboila C，Chin M，Higashi-Howard A，Shamash P，Wu Z，Stein NP，Abbott LF，AxelR。额叶皮层中气味值的瞬态和持续表示。(2020) Neuron, 108(1):209-224.e6. doi: 10.1016/j.neuron.2020.07.033. Epub 2020 Aug 21. PMID: 32827456; PMCID: PMC7886003
- Wu，Z.，Litwin-Kumar，A.，Shamash，P.，Taylor，A.，Axel，R。，＆Shadlen，M。N.在前后电路中取决于上下文的决策。(2020) Neuron, 106(2), 316-328.e6. doi: 10.1016/j.neuron.2020.01.034
- Beaulieu-Laroche, L., Toloza, E. H. S., Brown, N. J., & Harnett, M. T.Widespread and Highly Correlated Somato-dendritic Activity in Cortical Layer 5 Neurons.（2019）Neuron，103（2），235-241.e4。doi：10.1016/j.neuron.2019.05.014
- Dalmay T，ABS E，Poorthuis RB，Hartung J，Pu DL，Onasch S，Lozano YR，Signoret-Genest J，Tovote P，Gjorgjieva J，Letzkus JJ。A Critical Role for Neocortical Processing of Threat Memory.（2019）Neuron，104（6）：1180-1194.e7。doi：10.1016/j.neuron.2019.09.025。EPUB 2019 11月11日。PMID：31727549。
- Desjardins, M., Kılıç, K., Thunemann, M., Mateo, C., Holland, D., Ferri, C. G. L., … Devor, A.Awake Mouse Imaging: From Two-Photon Microscopy to Blood Oxygen Level–Dependent Functional Magnetic Resonance Imaging.(2019) Biological Psychiatry: Cognitive Neuroscience and Neuroimaging, 4(6), 533–542. doi: 10.1016/j.bpsc.2018.12.002
- Hutter G，Theruvath J，Graef CM，Zhang M，Schoen MK，Manz EM，Bennett ML，Olson A，Azad TD，Sinha R，Chan C，Chan C，Assad Kahn S，Gholamin S，Wilson S，Wilson C，Grant G，Grant G，He J He J，Weissman，Weissman，WeissmanIL，Mitra SS，Cheshier sh。Microglia are effector cells of CD47-SIRPα antiphagocytic axis disruption against glioblastoma.（2019）Proc Natl Acad Sci U S A.，116（3）：997-1006。doi：10.1073/pnas.1721434116。EPUB 2019 JAN2。PMID：30602457;PMCID：PMC6338872
- Jennings, J. H., Kim, C. K., Marshel, J. H., Raffiee, M., Ye, L., Quirin, S., … Deisseroth, K.Interacting neural ensembles in orbitofrontal cortex for social and feeding behaviour.(2019) Nature, 565(7741), 645–649. https://doi.org/10.1038/s41586-018-0866-8
- Lancaster, J. N., Thyagarajan, H. M., Srinivasan, J., Li, Y., Hu, Z., & Ehrlich, L. I. R.活细胞成像揭示了抗原呈递细胞子集对胸腺中心耐受性的相对贡献。（2019）自然通讯，10（1）。doi：10.1038/s41467-019-09727-4
- Marshel, J. H., Kim, Y. S., Machado, T. A., Quirin, S., Benson, B., Kadmon, J., … Deisseroth, K.Cortical layer-specific critical dynamics triggering perception.（2019）科学，365（6453）。doi：10.1126/science.aaw5202
- McIver, E. L., Atherton, J. F., Chu, H. Y., Cosgrove, K. E., Kondapalli, J., Wokosin, D., … Bevan, M. D.Maladaptive Downregulation of Autonomous Subthalamic Nucleus Activity following the Loss of Midbrain Dopamine Neurons.(2019) Cell Reports, 28(4), 992-1002.e4. doi: 10.1016/j.celrep.2019.06.076
- el,迈克耳逊:J。J . R。巴斯克斯,a . L。路德wig, K. A., & Kozai, T. D. Y.通过连续电刺激对皮质神经元的钙激活：频率依赖性，时间忠诚度和激活密度。(2019) Journal of Neuroscience Research, 97(5), 620–638. doi: 10.1002/jnr.24370
- Mitchell，D.E.，Martineau，é。，Tazerart，S。和Araya，R。Probing single synapses via the photolytic release of neurotransmitters.（2019）突触神经科学领域的前沿，11（7月）。doi：10.3389/fnsyn.2019.00019
- Solarana K, Liu J, Bowen Z, Lee HK, Kanold PO.Temporary Visual Deprivation Causes Decorrelation of Spatiotemporal Population Responses in Adult Mouse Auditory Cortex.(2019) eNeuro., 6(6):ENEURO.0269-19.2019. doi: 10.1523/ENEURO.0269-19.2019. PMID: 31744840; PMCID: PMC6901683
- Stocking, K. C., Vazquez, A. L., & Kozai, T. D. Y.带有光电效应介导的不受限制的超质碳纤维电极的皮质内神经刺激。（2019）IEEE生物医学工程交易，66（8），2402–2412。doi：10.1109/tbme.2018.2889832
- Stowell，R。D.，Sipe，G。O.，Dawes，R。P.，Batchelor，H。N.，Lordy，K。A.，Whitelaw，B. S.在觉醒状态下的去肾上腺素能信号传导抑制小鼠视觉皮层中的小胶质细胞监测和突触可塑性。（2019）自然神经科学，22（11），1782– 1792年。doi：10.1038/s41593-019-0514-0
- Tanimura，A.，Du，Y.，Kondapalli，J.，Wokosin，D.L。和Surmeier，D.J。胆碱能中神经元在帕金森氏病模型中扩增了纹状体间接途径神经元的丘脑纹状体激发。(2019) Neuron, 101(3), 444-458.e6. doi: 10.1016/j.neuron.2018.12.004
- Turi, G. F., Li, W. K., Chavlis, S., Pandi, I., O’Hare, J., Priestley, J. B., … Losonczy, A.Vasoactive Intestinal Polypeptide-Expressing Interneurons in the Hippocampus Support Goal-Oriented Spatial Learning.(2019) Neuron, 101(6), 1150-1165.e8. doi: 10.1016/j.neuron.2019.01.009
- Venkatesh, H. S., Morishita, W., Geraghty, A. C., Silverbush, D., Gillespie, S. M., Arzt, M., … Monje, M.Electrical and synaptic integration of glioma into neural circuits.(2019) Nature, 573(7775), 539–545. doi: 10.1038/s41586-019-1563-y
- Zhao, W., Garcia-Oscos, F., Dinh, D., & Roberts, T. F.Inception of memories that guide vocal learning in the songbird.（2019），科学，366（6461），83-89。doi：10.1126/science.aaw4226
- Abs, E., Poorthuis, R. B., Apelblat, D., Muhammad, K., Pardi, M. B., Enke, L., … Letzkus, J. J.Learning-Related Plasticity in Dendrite-Targeting Layer 1 Interneurons. (2018) Neuron, 100 (3), 684-699.e6. doi: 10.1016/j.neuron.2018.09.001
- -Clark, T. A., Fu, M., Dunn, A. K., Zuo, Y., & Jones, T. A.Preferential stabilization of newly formed dendritic spines in motor cortex during manual skill learning predicts performance gains, but not memory endurance.(2018) Neurobiology of Learning and Memory, 152, 50–60. doi: 10.1016/j.nlm.2018.05.005
- Kaelberer, M. M., Buchanan, K. L., Klein, M. E., Barth, B. B., Montoya, M. M., Shen, X., & Bohórquez, D. V.A gut-brain neural circuit for nutrient sensory transduction.(2018) Science, 361(6408). doi: 10.1126/science.aat5236
- Pho, G. N., Goard, M. J., Woodson, J., Crawford, B., & Sur, M.小鼠顶皮层中刺激和选择的任务依赖性表示。(2018) Nature Communications, 9(1). doi: 10.1038/s41467-018-05012-y
- Yan, Y., Peng, C., Arvin, M. C., Jin, X. T., Kim, V. J., Ramsey, M. D., … Drenan, R. M.Nicotinic Cholinergic Receptors in VTA Glutamate Neurons Modulate Excitatory Transmission.(2018) Cell Reports, 23(8), 2236–2244. doi: 10.1016/j.celrep.2018.04.062
- Yang，W。和Yuste，R。全息成像和神经活动的光刺激。（2018）当前神经生物学意见。Elsevier Ltd. doi：10.1016/j.conb.2018.03.006
- Zhang，Z.，Russell，L。E.，Packer，A.M.，Gauld，O.M。，＆Häusser，M。Closed-loop all-optical interrogation of neural circuits in vivo.(2018) Nature Methods, 15(12), 1037–1040. doi: 10.1038/s41592-018-0183-z
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