天涯在Vitro
Основные моменты
天涯在Vitro
The Ultima In Vitro Multiphoton Microscope System is the platform on which leading scientists have conducted breakthrough experiments in brain-slice studies related to neural signaling and neural network mapping. Ultima In Vitro provides simultaneous imaging and electrophysiology, plus uncaging and photostimulation for the complete stimulus and recording environment required for research by today’s neuroscientist.
„б
灵活的体系结构满足不同的要求
天涯在Vitro is the ultimate “slice rig," having provided the platform on which leading scientists have conducted breakthrough experiments in brain slices related to neural signaling and neural networks for more than ten years. The system provides the flexibility for the most demanding protocols, and is available on upright or inverted optical microscopes. Features include:
- 二次scan path that enables simultaneous imaging and photostimulation
- Visible, UV, and IR inputs for photostimulation
- Sub-micron positioning control for point photoactivation
- Sub-millisecond spirals for somal actiavtion
- 同步和触发以同时进行成像，光刺激和电生理学
- Integrated electrophysiology control and recording with up to 16 analog outputs/inputs
High-Resolution Multifield Imaging
The Atlas Imaging module in Prairie View software simplifies setup of 2D and 3D automated montages. X,Y boundaries are easily setup through a thumbnail display. Tiles not containing areas of interest can be turned off, and the Z range for 3D montages can be variable, allowing more efficient collection of data.
Pharmaceutical Studies with Tissue Explants
Tissue explants provide a higher throughput model for studying tissue response to pharmaceutical agents. The FLIM option allows measurement of metabolic status through measurement of fluorescent lifetimes.
Neuronal Signaling in Brain Slices
天涯在Vitro is the system of choice for studying neuronal signaling at the both the dendritic and neural network levels. When equipped with optional photostimulation galvanometers, Ultima In Vitro provides a complete optical workstation, allowing simultaneous imaging, photostimulation and electrophysiology recording.
Volumetric Imaging of Cell Preparations
天涯在Vitro provides high resolution and efficient volumetric imaging of cell cultures and cells in artificial matrices.
Спецификации
Specifications
| SCAN HEAD | |
| 扫描方法 | 匹配的6毫米剑桥仪表仪具有光栅和螺旋扫描功能 |
| Field of View | ~600 μm x ~600 μm with 20x objective |
| 扫描速度 | Raster scan: 1.65 fps at 512 x 512, >12 fps at 64 x 64 Spiral scan: 6 fps at 512 x 512, ~30 fps at 64 x 64 |
| Scan Customization | 具有用户定义的笔直，徒手和圆形（无限）线can具有随附的软件；从1-2048开始的每行扫描和每行扫描行的用户可定义像素；最多128倍扫描变焦；360°扫描旋转；点扫描 |
| Uncaging Option | 在同一扫描头上匹配的第二组匹配的3毫米剑桥仪表仪，以提供高精度可见或多光子激光样品消融 |
| High-Speed Imaging Option | 8 kHz resonant galvanometer; 30 fps at 512 x 512, >1300 fps at 512 x 8 region of interest |
| 共焦Option | Point scan: Automated pinhole confocal attachment with up to three PMTs Multipoint scan: Camera-based for high-speed imaging |
| 探测器 | |
| Reflected Non-Descanned | 1 - 4 hand-picked Hamamatsu Multi-Alkali PMTs; upgradeable to high-sensitivity Hamamatsu GaAsP PMTs |
| 传输非散布 | 1 - 2 hand-picked Hamamatsu Multi-Alkali PMTs; upgradeable to high-sensitivity Hamamatsu GaAsP PMTs |
| Dodt | Single Hamamatsu PMT for DIC-like image collection |
| Transmitted | Single Hamamatsu PMT for transmitted light image collection |
| 共焦 | Point scan: 1 - 3 hand-picked Hamamatsu Multi-Alkali PMTs; upgradeable to high-sensitivity Hamamatsu GaAsP PMTs Multipoint: EMCCD |
| Camera | Standard C2 camera port built into scan head for standard OLM image collection |
| OPTICAL INPUTS | |
| Multiphoton Laser | Ultima light path is optimized for multiphoton laser input from 690 - 1300 nm |
| Epifluorescence | Standard epifluorescence head included for fluorescence imaging |
| 可见激光 | Fibered laser inputs for visible laser introduction |
| Ultima Laser Rating | 1类（包含3B和4类激光器） |
| Helios Laser Rating | Class 3b |
| LED | LED模块的全场光活化 |
| 平台和自动化 | |
| Microscope | 尼康Ti-E倒和尼康Ni-E直立;Olympus BX51WI and BX61WI; Zeiss Axio Observer and Axio Examiner |
| Specimen Stage | Bruker Slim Stage for upright; ASI stage with piezo insert for inverted |
| Z-Focus | Internal Focus Motor for Ti-E, BX 61 and Zeiss; Bruker z-focus motor for BX51WI and Ni-E; Bruker z-piezo available on BX51WI, BX61WI and Ni-E |
| 软件 | |
| Prairie View Imaging | Software fully integrated with scan head for easy imaging; customizable scan settings for optimization of specimen excitation; integrated control of laser power and PMT high voltages |
| Z-Series | 轻松创建具有用户可自定义的切片号，步长和激光功率的深度堆栈 |
| T-Series | 轻松创建涉及Z系列和触发图像的复杂系列 |
| Stage Montage | Atlas Imaging simplifies setup and optimizes acquisition of 2D and 3D stage montages |
| Peripherals Integration | Wavelength and power control available for multiphoton and visible laser launches |
| Photoactivation | 用户定义的复杂激光空白点和区域 |
| Regions of Interest | User-defined regions for faster scanning capabilities |
| Brightness Over Time | Capable of collecting BOT information for user-defined regions over time and/or depth |
| Voltage Inputs/Outputs | Signal inputs and outputs for electrophysiological experiments, stimulus control, and synchronization with internal devices |
Аксессуары
天涯在Vitro Accessories and Options
- Provides capability for simultaneous photostimulation or uncaging with imaging
- Available for fast pulsed IR, UV or visible laser inputs
- Precise synchronization with image and external triggers
- Provides FLIM acquisition integrated with all raster scan galvo modes and ROIs
- Enables the inclusion of FLIM experiments in Z and T-Series
- 提供0.05μm精度的高速Z位置
- 聚焦在150μm的范围上
- Unique calibration enables continuous movement for no dead-time during z-series acquisitions
- Provides hardware and software components for imaging with two different multiphoton wavelengths simultaneously (with two lasers)
- Software options allow for interlaced or simultaneous excitation scanning
- Enables excitation of multiple probes with different excitation wavelengths without down-time for laser tuning
- Provides full-field access to the imaging axis for light stimulus introduction or widefield imaging
- Variable zoom capability
Вебинары
Recent Multiphoton Microscopy Webinars
→зы
Hear What Our Customers Have to Say
我们感谢Ultima硬件和软件的模块化性质。现在可用的系统范围从基本成像系统到双激光刺激多模式成像工作站。我们继续更新14个机器中的许多，包括我们十年的系统。对于新手所有者和重大软件更改而言，团队观众的连接和支持是一项巨大的资产。
