Trulive3D成像仪
ハイライト
Trulive3D成像仪
Optimized for fast 3D multi-sample volume imaging of delicate live specimen in their native 3D environment, the TruLive3D Imager is especially suited for multi-position imaging of small embryos (e.g. mouse), 3D spheroids, organoids, oocytes and more, enabling time-lapse, high-throughput imaging experiments. The optical concept, with dual-sided illumination and single-lens detection from below, enables fast acquisition speed, high-resolution imaging and minimal shadowing effects.
特長
Compact, Robust and Vibration-Free Design
The TruLive3D Imager's compact, robust and vibration-free design provides maximal stability even during long-term and high-throughput experiments.
Tailored to fit your lab bench, this class 1 laser system does not require any air table or vibration-compensation mechanism as all moving components are light-weight and balanced. Maximal stability of focus and thermal conditions are also guaranteed. The proprietary piezo-crawler stages ensure longevity and precision for a permanently accurate specimen positioning. Neither the images nor the quality of your sample is affected thanks to the unique and very gentle image-acquisition concept.
Deep and Crisp Imaging
照明and Detection
The TruLive3D Imager can achieve a resolution down to 255 nm in xy, enabling resolving subcellular structures in living samples free of phototoxic effects.
The system features dual-sided illumination and single-lens detection from below, enabling fast acquisition speed, high-resolution imaging, and minimal shadowing effects. A wide-field imaging option facilitates sample positioning.
两个Nikon CFI计划荧光10x W 0.3 Na浸入物镜镜片项目样品上的灯页。检测包括高数值孔径Nikon CFI APO 25X W 1.1 Na浸入物镜。根据您的实验需求，一个额外的放大率更换器可导致31.3倍和62.5倍的总放大倍率，以进行现场视图和采样调整。
Native and Natural
The TruLive3D Imager is equipped with environmental control. Temperature can be adjusted between 20–37°C for optimal incubation conditions.
此外，Trulive3D成像仪还提供精确稳定的环境控制（即CO2, O2, N2，湿度）。CO的不同组件的气体浓缩范围在0-15％之间2, 1–21 % for O2,and 20–99 % for H2O(湿度)。气体加湿器c提供反馈ontrol for precise regulation.
The TruLive3D Imager is optimized for fast 3D multi-sample imaging of delicate live specimen in their native 3D environment.
多路复用
Large Sample Holder
The extended sample chamber in the TruLive3D Imager fits a large sample holder (length=75 mm) which can accommodate tens to hundreds of samples. It is designed for multi-position imaging of small embryos (e.g. zebrafish, Drosophila or mouse), 3D spheroids, oocytes and more.
The FEP foil lining the holder serves dual functions, a “curved coverglass” and a physical barrier between the immersion medium and sample medium.
TruLive3D Dish
The new TruLive3D Dish series of disposable sample holders seamlessly integrate into the system and allow testing different media conditions in one experiment.
The new ready-to-use TruLive3D Dishes are fully compatible with the TruLive3D Imager. The possibility to line up three disposable dishes extends the capacities of the system to enable the parallel imaging of samples grown under different experimental conditions.
TruLive 3D Imager Design
Lux DATA - A Comprehensive Data Processing and Storage Solution
Cutting-edge biological imaging like light-sheet microscopy, but also super-resolution or two-photon imaging, generates a vast amount of data required to provide researchers with all the relevant information about their samples. However, storage, transfer, and processing of the data remain a challenge.
アプリケーション
实时样本应用
从下面的客户中浏览一些应用程序数据。研究人员正在以多种方式使用Trulive3D成像仪，包括胚胎发生和发育生物学的研究，器官，细胞培养物，神经生物学和神经发育，植物等。
Pancreatic Sphere
hESC-derived pancreatic spheres. Imaging on the TruLive3D Imager enables collecting information of several samples in one experiment. Visualization: Imaris (Bitplane)
提供：
Yung Hae Kim
Grapin-Botton Group, MPI-CBG
Dresden, Germany
仕様
Specifications
| 照明 | Detection |
Effective Magnification |
Field of View | 像素是ze | Optical Resolution |
| 10x / 0.3 NA | Nikon 25x / 1.1 NA | 31.3x 62.5x |
420 µm 210 µm |
208 nm 104 nm |
255 nm |
照明Optics
- Chromatic correction from 440 to 660 nm
- Light-sheet generation by beam scanning
- Flexible light-sheet thickness (2 µm to 6 µm)
- Motorised ND filter set (90%, 10%, 1%) for high flexibility in using low laser power
- Two Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lens
检测光学
- One Nikon CFI Apo 25x W 1.1 NA water immersion objective lens
- Two spectral detection channels, each equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions) Filters adapted to the selected laser lines
- Three motorised dichroic mirror positions for simultaneous dual channel acquisition
- Motorised collar ring controlTwo high-speed sCMOS cameras Hamamatsu Orca Flash 4.0 V3
- Maximum frame rate >80 fps at full frame (2048 × 2048 pixels of 6.5 µm × 6.5 µm size) and up to 500 fps at subframe cropping
- Peak quantum efficiency (QE): 82% @ 560 nm
アクセサリー
Accesories
Trulive3D菜肴
TrueLive3D Imager Sample Holder
ソフトウェア
软件
Luxendo的直观用户界面提供了多维实验的简单设置和执行，而实时控制器由嵌入式控制器处理，以确保与PC的性能波动无关。
Precise timing control of all connected devices is a prerequisite for reliable experimental outcomes. Full control of data streaming to storage as well as GPU-supported image processing further complements the overall performance.
Electronics, Microscope Software and Computer
- Embedded microscope software with an open communication interface: documented API, TCP/IP-based communication
- Flexible GUI for interactive microscope control and experiment design
- Computer with 256 GB RAM, Intel dual 8 core CPU
- High-speed RAID controller for data streaming, 8×4 TB local storage in RAID 0
- GeForce RTX 3070 graphics card
- 10 Gbit/s on-board Ethernet port
- 1 UHTV 43 inch display
