Trulive3D成像仪
伪造的侯爵夫人
Trulive3D成像仪
Trulive3D成像仪在其本机3D环境中针对精致的活标本的快速3D多样本体积成像进行了优化，特别适合小胚胎（例如小鼠），3D球体，器官，卵母细胞等的多位置成像，以实现时间 -失误，高通量成像实验。具有双面照明和单镜头检测的光学概念可以快速获取速度，高分辨率成像和最小的阴影效果。
caractéristiques
紧凑，健壮和无振动设计
Trulive3D成像仪的紧凑，健壮和无振动的设计即使在长期和高通量实验中也可以提供最大稳定性。
Tailored to fit your lab bench, this class 1 laser system does not require any air table or vibration-compensation mechanism as all moving components are light-weight and balanced. Maximal stability of focus and thermal conditions are also guaranteed. The proprietary piezo-crawler stages ensure longevity and precision for a permanently accurate specimen positioning. Neither the images nor the quality of your sample is affected thanks to the unique and very gentle image-acquisition concept.
Deep and Crisp Imaging
照明and Detection
Trulive3D成像仪可以在XY中达到低至255 nm的分辨率，从而在没有光毒性效应的生物样品中解决亚细胞结构。
The system features dual-sided illumination and single-lens detection from below, enabling fast acquisition speed, high-resolution imaging, and minimal shadowing effects. A wide-field imaging option facilitates sample positioning.
两个Nikon CFI计划荧光10x W 0.3 Na浸入物镜镜片在样品上投影。检测包括高数值孔径Nikon CFI APO 25X W 1.1 Na浸入物镜。根据您的实验需求，一个额外的放大变换器可导致31.3倍和62.5倍的总放大倍率，以进行现场视图和采样调整。
Native and Natural
Trulive3D成像器配备了环境控制。可以在20–37°C之间调节温度以达到最佳的孵育条件。
此外，Trulive3D成像器还提供精确稳定的环境控制（即CO2, O2, N2，湿度）。CO的不同组件的气体浓缩范围在0-15％之间2, 1–21 % for O2，，H和20％的H2O(湿度)。气体加湿器c提供反馈ontrol for precise regulation.
Trulive3D成像仪已针对其本机3D环境中精致的活标本的快速3D多样本成像进行了优化。
多路复用
大型样品支架
The extended sample chamber in the TruLive3D Imager fits a large sample holder (length=75 mm) which can accommodate tens to hundreds of samples. It is designed for multi-position imaging of small embryos (e.g. zebrafish, Drosophila or mouse), 3D spheroids, oocytes and more.
The FEP foil lining the holder serves dual functions, a “curved coverglass” and a physical barrier between the immersion medium and sample medium.
TruLive3D Dish
新的Trulive3D Dish Dish系列一次性样品持有人无缝集成到系统中，并允许在一个实验中测试不同的介质条件。
新的即用Trulive3D菜肴与Trulive3D成像仪完全兼容。排列三个一次性菜肴的可能性扩大了系统的能力，以使在不同的实验条件下生长的样品的平行成像。
Trulive 3D成像器设计
Lux DATA - A Comprehensive Data Processing and Storage Solution
Cutting-edge biological imaging like light-sheet microscopy, but also super-resolution or two-photon imaging, generates a vast amount of data required to provide researchers with all the relevant information about their samples. However, storage, transfer, and processing of the data remain a challenge.
Applications
实时样本应用
从下面的客户浏览一些应用程序数据。研究人员正在以多种方式使用Trulive3D成像仪，包括胚胎发生和发育生物学，器官，细胞培养物，神经生物学和神经发育，植物等方面的研究。
胰腺球
hESC-derived pancreatic spheres. Imaging on the TruLive3D Imager enables collecting information of several samples in one experiment. Visualization: Imaris (Bitplane)
提供：
Yung Hae Kim
Grapin-Botton Group，MPI-CBG
德累斯顿，德国
规格
Specifications
| 照明 | Detection |
有效放大 |
Field of View | 像素是ze | Optical Resolution |
| 10x / 0.3 na | 尼康25x / 1.1 NA | 31.3x 62.5x |
420 µm 210 µm |
208 nm 104 nm |
255 nm |
照明光学
- 从440到660 nm的色度校正
- 光束扫描的灯页生成
- Flexible light-sheet thickness (2 µm to 6 µm)
- Motorised ND filter set (90%, 10%, 1%) for high flexibility in using low laser power
- Two Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lens
检测光学
- One Nikon CFI Apo 25x W 1.1 NA water immersion objective lens
- Two spectral detection channels, each equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions) Filters adapted to the selected laser lines
- 同时双通道获取的三个电动二色性镜像位置
- Motorised collar ring controlTwo high-speed sCMOS cameras Hamamatsu Orca Flash 4.0 V3
- 最大帧速率> 80 fps在全帧（2048×2048像素为6.5 µm×6.5 µm）和最多500 fps，在副构造时
- 峰量效率（QE）：82％ @ 560 nm
访问
Accesories
Trulive3D菜肴
truelive3D成像器样品持有人
逻辑
软件
Luxendo的直观用户界面提供了多维实验的简单设置和执行，而实时控制器则由嵌入式控制器处理，以确保与PC的性能波动无关。
Precise timing control of all connected devices is a prerequisite for reliable experimental outcomes. Full control of data streaming to storage as well as GPU-supported image processing further complements the overall performance.
Electronics, Microscope Software and Computer
- Embedded microscope software with an open communication interface: documented API, TCP/IP-based communication
- Flexible GUI for interactive microscope control and experiment design
- 带有256 GB RAM的计算机，Intel Dual 8 Core CPU
- High-speed RAID controller for data streaming, 8×4 TB local storage in RAID 0
- GeForce RTX 3070 graphics card
- 10 Gbit/s板载以太网端口
- 1 UHTV 43 inch display
