命令的最终表现
新的多尺度X射线纳米仪Skyscan 2214涵盖了单个仪器中最宽的物体大小和空间分辨率。它为所有类型的生物样品(包括矿化组织,软组织,植物,昆虫,古生物学样本等)提供了独特的3D成像和精确建模的可能性。
该仪器允许对物体的内部微观结构进行扫描和3D非破坏性重建,直径至300mm,以及用于小样品的亚微米分辨率。
The system contains an "open type" transmission X-ray source with <0.5 microns spot size and a diamond window. It can be equipped with up to four different X-ray detectors for maximum flexibility: flat-panel for large objects, 11Mp cooled CCD with wide field of view, 11Mp cooled CCD with medium field of view, and 8Mp cooled CCD for highest spatial resolution. Automatic variable acquisition geometry and phase-contrast enhancement allows best possible quality in relatively short scanning time.
Skyscan 2214由3D.Suite补充。这个全面的软件套件涵盖了GPU加速重建,2D/ 3D形态分析以及表面和音量渲染可视化
The SKYSCAN 2214 uses a latest generation open-type X-ray source. The source offers true spatial resolution below 500 nm, an X-ray energy up to 160 keV and source power up to 16 W. The source is practically maintenance-free with an extremely easy pre-aligned filament replacement procedure.
Skyscan 2214具有带有钻石窗口的开放型(泵送)纳米焦X射线源。它产生的X射线束从20 kV到160 keV,并提供两种类型的阴极。钨(W)阴极在最高160 kV的整个加速电压下运行,并提供一个斑点尺寸降至800 nm。甲状腺六角杆(LAB6)阴极可用于将电压从20 kV加速至100 kV,并提供小于500 nm的X射线束的斑点,以实现成像和3D重建方面的最高分辨率。JIMA分辨率模式表明500 nm结构可以轻松解析。
For long-term stability of the focal spot size and position of the emission point, the X-ray source is equipped with a liquid cooling system which contains a re-circulator providing precise temperature stability of the cooling fluid.
The SKYSCAN 2214 can be equipped with up to four X-ray cameras for ultimate flexibility: three CCD cameras with different resolution and field of view and one large-area flat panel detector. All cameras can be selected with a single mouse click. The different CCD cameras can be retro-fitted at any point of time during the system’s lifetime.
All three CCDs can take images in the central beam position and in two offset positions to double the field of view. The images in the two offset positions are automatically stitched together with compensation of the shifts and possible intensity differences.
使用具有小像素尺寸的CCD检测器可以将高分辨率成像和3D重建的扩展为大对象。内置检测器的灵活性可以根据对象大小和密度调整视野和空间分辨率。来自兴趣量的高级重建提供了对大分子的选定部分具有高分辨率的扫描,而不会损害图像质量。
Additionally, the field of view can be increased horizontally and vertically by using offset camera positions and vertical object movement.
The high-precision object stage of the SKYSCAN 2214 supports objects up to 300 mm diameter and 20 kg in weight. The air-bearing rotation motor allows precise rotation of objects at very high accuracy, and the integrated micro-positioning stage guarantees a perfect sample alignment.
The SKYSCAN 2214 has a large and easily accessible sample chamber to allow scanning of big objects as well as mounting of optional stages. On top plenty of space is available for peripheral equipment.
Bruker材料测试阶段旨在执行高达4400 N的压缩实验和最高440 N的拉伸实验。所有阶段都可以通过系统的旋转阶段自动通信,而无需任何电缆连接。使用提供的软件,可以设置计划的扫描实验。
Bruker's heating & cooling stages can reach temperatures of up to +80 °C or 30 °C below ambient temperature. Just like the other stages, no extra connections are needed, and there is an automatic recognition of the stage. Using the heating & cooling stages, samples can be examined under non-ambient conditions, to evaluate the effect of temperature on the sample’s microstructure.
Skyscan 2214与Deben的阶段完全兼容。使用随附的适配器,可以简单地将Deben阶段放在Skyscan 2214的旋转阶段。
The SKYSCAN 2214 provides ultimate nanoCT imaging performance without compromise. Four cameras are fitted – one large format 6 megapixel cMOS flat panel for imaging up to high voltages and large skeletal or dental samples, and a range of three small format detectors for the optimal choice of resolution and x-ray energy window for every conceivable bone sample scan.
Submicron voxel scans provide crystal clear resolution of bone osteocyte lacunae and micro-scale mineralization structure, as well as architecture of biomaterial scaffolds at nano-scale resolution.
相位检索(Paganin)提供了对迄今为止研究的骨微矿化模式分析的新维度。
Orthopedic research with sheep, primate or similar models is handled with the 14cm scan field of view and 160kV x-ray source. Realize your orthopedic and biomechanical research goals with mechanical testing and temperature control stages.
Morphometry with comprehensive 3D and 2D parameters, with densitometry including BMD calibration references over the preclinical size range. Advanced 3D image analysis functions include 3D registration, adaptive thresholding, Euler connectivity, fractal, anisotropy and stereology, filtering, Boolean logical operators and much more.
Ex vivo nano-CT scanning of biological tissues, with sub-micron pixel size, is like histology or electron microscopy but in true, deep 3D - a fantastic method to show internal structures non-destructively. Contrast agents or chemical drying can improve the image quality by further enhancing or differentiating tissue densities. The SKYSCAN 2214 is playing a leading role in the emergence of a new imaging discipline – microCT histology and histomorphometry.
The unmatched versatility of this system ensures that each sample will be scanned with optimized parameters and the best resolution and contrast.
Four cameras are fitted – a large format 6 megapixel flat panel for imaging up to high voltages and large tissue samples, and a range of three small format cameras for the optimal choice of resolution and x-ray energy window for every conceivable sample scan.
Comprehensive 3D image analysis capability including morphometry and densitometry, 3D registration, segmentation and advanced image processing methods.
MicroCT is exceptionally good for the visualization of the finest details of internal biological structures. This imaging method creates a nano-scale 3D x-ray attenuation map without harming or destroying the scanned object. Practically all biological tissues can be visualized and analyzed with little or no need of special sample treatment. Submicron voxel resolution allows imaging of small insect and plant or seed structures with powerful magnification and rich detail.
扫描室中的空间以及旋转阶段的精度确保可以扫描所有类型的样品,从小斑马鱼和保存的动物学和植物样品到盆栽的植物和岩石填充的化石。
Four cameras are fitted – a large format 6 megapixel flat panel for imaging up to high voltages and large samples, and a range of three small format cameras for the optimal choice of resolution and x-ray energy window for every conceivable biological sample scan.
该软件套件可以通过全面的3D和2D参数实现形态计量学,高级3D图像分析功能包括3D注册,自适应阈值,Euler连接,分形,各向异性和立体,过滤,Boolean逻辑运算符等。
3D model of an entire bee head (left), and virtually cut to expose internal structures (right), scanned at 1.4 µm voxel size.
3D model of a toothpick, scanned at nanoscale resolution.
Volume rendered image of a subvolume of dentin from a human molar, scanned at 350 nm voxel size. The model is virtually cut to show the dentine tubules running through the dentin in blue.
Orthogonal slices though a mouse distal tibia showing the osteocyte lacunae, scanned at 1 µm voxel size.
Volume render of a piece of wood, scanned at 200 nm voxel size (left) with the vascular network color-coded for local thickness (right).
3D model of a sheep bone with 2 large titanium implants.
Mouse lungs scanned at 3 µm voxel size after chemical drying.
3D Volume rendered image of a medieval human skull from Thuringia, scanned at 50 μm voxel size (Sample from the Friedrich-Schiller University Jena, Germany).
Cross-section through a Brassica seed, scanned at nanoscale resolution.
Feature |
规格 |
益处 |
X-ray source |
20-160 kV 16 W max. |
User exchangeable filament 优化最大功率(W)或最大分辨率(LAB6) Rotatable diamond window for maximum lifetime |
X-ray detector |
6 MP活动像素平板板 11Mp large format cooled CCD 11Mp mid format cooled CCD 8 Mp hi-res cooled CCD |
多种像素和检测器的大小可以在检测器分辨率,覆盖范围和计数统计数据之间平衡 可提供1、2、3或4个检测器 Field upgradable to add detectors |
图像格式 |
Up to 8000 x 8000 x 2300 pixels after a single scan |
User selectable image size allows balance of dataset size and needed resolution 数据收集后软件允许裁员 |
决议 |
60 nm最小的像素大小 |
Simple graphics control for optimizing experiment resolution based on selected detector, sample and detector distance Adjustable source focus size to balance maximum power and resolution |
定位准确性 |
<50 nm for rotation |
Air bearing sample stage provides smooth rotation Simple chuck style mounting of sample posts 高级材料研究阶段的机械和电界面bob综合游戏 |
Maximum Object Size |
300 mm in diameter (140 mm scanning size) 400 mm in length 最大物体重量20公斤 |
Both the power and room to scan large samples 靠近源的小样品的精确定位以最大化放大倍率 |
Dimensions | W 1800 mm x D 950 mm x H 1680 mm Weight: 1500 kg |
Efficiently designed to optimize use of lab space 源maintenance access via interlocked large sliding door |
Bruker XRM解决方案包括收集和分析数据所需的所有软件。具有用户指导参数优化的直观图形用户界面支持专家和新手用户。通过使用最新的GPU动力算法,重建时间大大减少。CTVOX,CTAN和CTVOL结合在一起,形成了一个强大的软件套件,用于模型的定性和定量分析。
Measurement Software:
SKYSCAN 1272 – Instrument control, measurement planning and collection
重建软件:
NRECON – Transforms the 2D projection images into 3D volumes
Analysis Software:
DATAVIEWER– Slice-by-slice inspection of 3D volumes and 2D/3D image registration
CTVOX– Realistic visualization by volume rendering
CTAN– 2D/3D image analysis & processing
ctvol– Visualization of surface models to export for CAD or 3D printing
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