QuVi SPIM
高亮
QuVi SPIM
Quvi Spim是一种高度通用的直立，双视图和双色灯页显微镜，用于生存，固定和清除样品的3D成像。基于我们健壮，稳定的系统平台，它将双视图的采集与高精度，长途旅行范围阶段相结合。在亚细胞各向同性分辨率下，针对长期的高通量成像进行了优化的QUVI SPIM。它具有简单的示例安装以及直观的软件和硬件概念。
功能
同时进行双视或双通道获取
QUVI SPIM将双重视图与双重检测通道相结合，以实现生命标本和清除样品的大规模3D成像。该系统可以在生活和清除的组织成像之间快速而简单的过渡。
Typical applications of the microscope include living or fixed and cleared brain slices, long-term imaging of 3D cell culture models (spheroids, organoids, tumoroids), imaging of conventional cell culture in high throughput, and even functional (e.g. calcium) imaging.
优化的稳定性
可以配备QuVi SPIM环境control. Temperature can be adjusted between 20–37°C for optimal incubation conditions.
此外，QUVI SPIM还提供精确稳定的环境控制（即CO2，o2，n2, and humidity). Gas-concentration for the different components ranges between 0–15 % for CO2，O的1-21％2, and 20–99 % for H2O(湿度)。气体加湿器offers feedback control for precise regulation.
用户友好的软件和硬件
硬件
它的新型舞台设计允许使用SBS格式板，并通过其快速负载功能提供了简单的样品可访问性。由于客观镜头是可以交换的，因此可以成像各种不同尺寸的样品。
Software
Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.
许多设计功能使Quvi Excell
直立，双重视图和双色
The QuVi SPIM combines easy accessibility with high throughput and well-controlled sample conditions, e.g. to perform screening experiments or biophysical methods such as FLIM and FCS.
Typical applications of the microscope include living or fixed and cleared brain slices, long-term imaging of 3D cell culture models (spheroids, organoids, tumoroids), imaging of conventional cell culture in high throughput, and even functional (e.g. calcium) imaging.
照明和检测
QUVI SPIM可以在3D中达到340 nm的分辨率，从而实现了实时成像，没有光毒性效应。
QUVI具有双侧交替的照明/检测概念，并提供了样本的两个正交视图。
照明光学元件包括通过横梁照射单元后扫描梁以进行长度和直径调节，包括光片生成。两个Nikon 40x 0.8 NA浸入物镜镜片交替投影样品上的两个正交灯表。可扩展和可升级的照明设置可允许多达六个激光器范围。无对齐的灯箱为每条线提供40MW的功率输出。为了检测，使用相同的客观镜头。
配备10个位置高速滤光轮的两个光谱检测通道和一个二分色镜更换器可同时同时进行双通道成像。另外，可以顺序获取两个以上的通道。来自Hamamatsu的强大SCMOS ORCA 4.0 V3摄像机非常适合需要高检测效率，定量和速度的实验。
Device Design
The QuVi SPIM's compact, vibration-free and robust design provides maximal stability during your long-term experiments.
为适合您的实验室长凳而定制的该类别1激光系统不需要任何空气桌或振动补偿机构，因为所有移动组件都是轻巧且平衡的。还保证了焦点和热条件的最大稳定性。专有的压电驱动器阶段可确保长寿和精确度，以实现永久准确的标本定位。样品的图像和自然生长行为都不受温和图像记录的影响。
Quvi Spim设计
Lux DATA - A Comprehensive Data Processing and Storage Solution
尖端的生物成像，例如灯页显微镜，以及超分辨率或两光子成像，它们会产生大量数据，以为研究人员提供有关其样品的所有相关信息。但是，数据的存储，传输和处理仍然是一个挑战。
应用
QuVi Applications
研究人员正在以多种方式使用QUVI SPIM，包括研究胚胎和发育生物学，类器官，细胞培养物，神经生物学和神经发育，植物等。从下面的客户浏览各种应用程序数据，或访问所有Bruker LightSeet Solutions的广泛画廊。
清除的小鼠淋巴结
BABB清除的小鼠淋巴结。高内皮静脉（红色）和自动荧光（绿色）以可视化周围的组织。成像在Quvi Spim上。
Courtesy of:
Jens Stein
University of Bern
伯尔尼，瑞士
3D细胞培养
3D culture system of human primary cells imaged on the QuVi SPIM.
Courtesy of:
亚森·阿巴斯（Yassen Abbas）
Turco Lab, University of Cambridge
Cambridge, UK
C. elegans Lineage Tracing
组蛋白标记（H2A :: MCHERRY，紫色）和膜标记（CND-1 :: GFP，Green）用于在秀丽隐杆线虫中进行谱系追踪。成像在Quvi Spim上。
Courtesy of:
Zhirong Bao
The Zhirong Bao Lab
纪念斯隆开氏癌中心（MSKCC）
Ney York, USA
Microglia Movement
Microglia movement in zebrafish. The vascular system is labeled with a cyan marker and microglia with a yellow one. Imaged on the QuVi SPIM at 2 FPS for 20 min. Two orthogonal views fused and max. project.
Courtesy of:
N. Norlin, F. peri
European Molecular Biology Laboratory (EMBL)
Heidelberg, Germany
规格
规格
| Illumination | Detection |
有效放大 |
Field of View | 像素大小 | Optical Resolution |
| 40X / 0.8 NA | 40X / 0.8 NA | 40.0x |
330 µm | 160 nm |
340nm |
照明光学
- 从440到660 nm的色度校正
- 光束扫描的灯页生成
- 柔性浅层厚度（2 µm至6 µm）
- 两个Nikon CFI APO 40X W 0.8 NA NA NIR浸入物镜镜头
Detection Optics
- 两个Nikon CFI APO 40X W 0.8 NA NA NIR浸入物镜镜头
- 3rd objective lens CFI Plan Achromat 4x 0.1 Air
- Dual-side alternating illumination/detection concept
- 2spectral detection channels, each equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions)
- Filters adapted to the selected laser lines
- 2high-speed sCMOS cameras Hamamatsu Orca Flash 4.0 V3
- Maximum frame rate >80 fps at full frame (2048 × 2048 pixels of 6.5µm × 6.5 µm size) and up to 500 fps at subframe cropping
- 峰量效率（QE）：82％ @ 560 nm
- 配备Grasshopper3 5.0MP摄像头的第三个检测路径可传输光检测
阶段
- 高精度XYZ阶段
- 独特的快速载荷功能，SBS板兼容
- Easy access from above for sample mounting, injections, etc.
- 环境控制单元可选，具有精确控制温度（范围20-37°C），湿度，O2和co2
激光组合器
- Maximum of 6 laser wavelengths (405, 445, 488, 515, 532, 561, 594, 642, and 685 nm with 40 mW each before fiber)
- 快速调制（MHz范围）和高消光比
软件
Software
Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.
Precise timing control of all connected devices is a prerequisite for reliable experimental outcomes. Full control of data streaming to storage as well as GPU-supported image processing further complements the overall performance.
电子，显微镜软件和计算机
- Embedded microscope software with an open communication interface: documented API, TCP/IP-based communication
- 用于交互式显微镜控制和实验设计的灵活GUI
- Computer with 128 GB RAM, Intel dual 8 core CPU
- 用于数据流的高速RAID控制器，RAID O中的8×4 TB本地存储
- GeForce RTX 2080图形卡
- 10 Gbit/s板载以太网端口
- 1 UHTV 40 inch display
