我nVi SPIM Family
我nVi SPIM Products
高亮
我nverted View
The optical configuration combined with the fast acquisition speed of the InVi SPIM enables 3D reconstruction, tracking of cellular and subcellular structures, and morphological analysis in real-time.
Typical applications of the microscope include dynamic processes in mammalian cell culture applications and even live imaging of intact and living plant models.
Advanced Illumination Module
The Advanced Illumination Module (AIM) is a modular extension that expands the capabilities of the InVi SPIM: while maintaining the ease-of-use and stability of the system, it adds tailorable, interactive adaptability of the beam shape to suit the highly specific requirements of your sample.
Adding a choice of various beam patterns – classical static Gaussian light-sheets, scanned Gaussian beams, Bessel beams, and optical lattices, or even customized shapes – provides unique flexibility to optimize your bioimaging experiments.
You choose what gives you the best results for your 3D high-resolution imaging experiment – a large field of view, high speed, or optimal spatial resolution.
Low Phototoxicity and Photobleaching
Dedicated to live imaging, the Luxendo InVi SPIM is a microscope that has been optimized for long-term 3D fluorescence imaging of living specimens.
Maximized photo-efficiency, and short illumination times enable long-term imaging without harming live specimens.
环境控制
The InVi SPIM is equipped with environmental control. Temperature can be adjusted between 20–37°C for optimal incubation conditions.
我n addition, the InVi SPIM also provides precise and stable environmental control (i.e. CO2, O2, N2，湿度）。CO的不同组件的气体浓缩范围在0-15％之间2, 1–21 % for O2and 20–99 % for H2O (humidity). The gas humidifier offers feedback control for precise regulation.
Sample Holder
The V-shaped, 3 cm long sample holder is placed inside of the chamber. It is covered with FEP foil allowing the physical separation of the sample from the chamber, without influencing imaging properties. Its reduced size ensures the use of small sample medium volume.
Different strategies can be used to place your sample in the holder for imaging. 2D cells can be grown directly on the foil as in a “curved coverglass”. 3D spheroids or small embryos can be dropped into the trough and held by gravitation, allowing multiple samples to be arranged for multi-position imaging.
The minimization of required specimen medium further leverages its functionality.
功能
倒置，快速和灵活
Dedicated to live imaging, the InVi SPIM offers easy access to the sample chamber and control of environmental conditions for long-term 3D fluorescence imaging of living specimen. Furthermore the InVi SPIM is much faster than a spinning disk or point scanning confocal microscope with much less photons.
我t is perfectly suited for a great variety of samples and applications ranging from cell culture to early embryos (e.g. Drosophila and Zebrafish).
我llumination and Detection
The InVi SPIM can achieve a resolution down to 255 nm in xy, enabling resolving subcellular structures in living samples free of phototoxic effects.
The system features one-sided illumination and single-lens detection, enabling fast acquisition speed, high-resolution imaging. A wide-field imaging option facilitates sample positioning.
One Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lenses project the light-sheet on the sample. Detection includes a high numerical aperture Nikon CFI Apo 25x W 1.1 NA water immersion objective lens. An additional magnification changer results in 31.3x and 62.5x total magnification for field view and sampling adjustment according to your experimental needs
The expandable, alignment-free light source allows a range of up to six lasers (405, 445, 488, 515, 532, 561, 594, 642, and 685 nm with 40 mW each before fiber).
检测光学元件可用于快速，高分辨率和非常敏感的成像。配备10个位置高速滤光轮的两个光谱检测通道和电动二角镜更换器同时实现双通道成像。另外，可以顺序获取两个以上的通道。来自Hamamatsu的强大SCMOS ORCA 4.0 V3摄像机非常适合需要高检测效率，定量和速度的实验。
Compared to other techniques (e.g. confocal laser scanning and spinning disc microscopy), light-sheet microscopy enables the acquisition of a higher number of time points while minimizing photo-damaging effects. A deeper insight into dynamic biological processes and fast movement tracking can be achieved.
Device Design
The InVi SPIM’s compact, vibration-free and robust design provides maximal stability during your long-term experiments.
Tailored to fit your lab bench, this class 1 laser system does not require any air table or vibration-compensation mechanism as all moving components are light-weight and balanced.
Maximal stability of focus and thermal conditions are also guaranteed. The proprietary piezo-crawler stages ensure longevity and precision for a permanently accurate specimen positioning. Neither the images nor the natural growth behaviour of your sample are affected by the gentle image recording.
应用
附件
Accesories
Trulive3D菜肴
在德国不提供Invi Spim产品家族。
我nVi SPIM Sample Holder for TruLive3D Dish
Not available in Germany.
我nVi SPIM Standard Sample Holder
Not available in Germany.
软件
软件
Luxendo的直观用户界面提供了多维实验的简单设置和执行，而实时控制器则由嵌入式控制器处理，以确保与PC的性能波动无关。
Precise timing control of all connected devices is a prerequisite for reliable experimental outcomes. Full control of data streaming to storage as well as GPU-supported image processing further complements the overall performance.
Electronics, Microscope Software and Computer
- Embedded microscope software with an open communication interface: documented API, TCP/IP-based communication
- Flexible GUI for interactive microscope control and experiment design
- Computer with 256 GB RAM, Intel dual 8 core CPU
- High-speed RAID controller for data streaming, 8 × 4 TB local storage in RAID O
- GeForce RTX 3070 graphics card
- 2 x 10 Gbit/s, 2 x 1 Gbit/s, optional 2x optical port on-board Ethernet port
- 43 inch 4k UHD display