NanoWizard 4 XP BioScience
ハイライト
NanoWizard®4XP Bioscience
The NanoWizard 4 XP BioScience atomic force microscope combines atomic resolution and fast scanning with rates of up to 150 lines/sec and a large scan range of 100µm in one system. It is designed to provide highest mechanical and thermal stability on inverted optical microscopes during long term experiments on samples ranging from single molecules to living cells and tissues.
特長
High-resolution imaging with extreme performance
The NanoWizard 4 XP BioScience comes with:
- PeakForce Tapping®用于简单成像
- Fast Scanning optionwith up to 150 lines/sec
- NestedScanner技术for high-speed imaging of surface structures up to 16.5µm with outstanding resolution and stability
- New tiling functionality用于大型样品区域的自动映射
- V7 Softwarewith revolutionary new workflow-based user interface
- DirectOverlay™ 2 software与高级荧光显微镜平台的完美集成和数据相关性
- Vortis™2控制器用于高速信号处理和最低噪声水平
Automated mapping of large sample areas with new tiling functionality
Hybridstage™或电动精确阶段通过直接访问大型样品区域，可以自动移动到选定位置，网格和映射区域，从而改变实验。
Begin with the DirectOverlay 2 optical calibration, and then select a region for optical tiling up to millimeters in size.
把wh自动精确的电机运动ole sample into view, making it easy to select regions and features for further investigation. A single click navigates from point to point or MultiScan experiments automate a sequence of measurements at selected points.
The images show living Vero cells in cell culture medium at 37°C in the PetriDishHeater™ . [2] - [5] Optical tiling with 5×6 phase contrast images covering a 630µm×450µm region. - Zoom into region scanned with AFM showing 100μm×100μm scan (height range 5μm) and inset 15μm×15μm (height range 2μm) scan topography images using PeakForce Tapping. The feedback correction signal images highlight the surface membrane features, particularly in the zoomed image. Microvilli dominate the center of the cell, with membrane ruffles at the cell boundary.
Perfect optical integration provides true correlative microscopy
The images show NanoWizard 4 XP setups on Zeiss LSM 880 confocal microscope with Airyscan [1] , with Upright Fluorescence Microscope (UFM) Kit for tissues or other large samples such as organs [2] , with BioMAT Workstation for high NA optics and Zeiss Axio Imager [3] , with TopviewOptics [4] and on on Olympus with PicoQuant MicroTime 200 STED [5].
Outstanding quantitative data from molecules, cells and tissues
QI™ Advanced, based on real force curves, offers both astounding speed and resolution for applications ranging from single molecules to living cells. The quantitative data allows precise and fast analysis of mechanical or biochemical interactions, e.g., localization of binding sites, directly overlaid with fluorescent labelling and topography with Molecular Recognition Imaging. Advanced batch processing options include multiple models for modulus fitting and can reveal surface topography at zero force with Contact Point Imaging (CPI).
The image shows single cell force spectroscopy measurements using the CellHesion module with an increased z range of 100 µm, showing the detachment force curves of a single A549 cell from fibronectin (FN) and from bovine serum albumin (BSA) coated culture dishes. Note, that the detachment of the cell from fibronectin results in a very large pulling range of 77 µm.
Key Features
- Fast Scanning optionwith up to 150 lines/sec for tracking dynamic processes
- Now with Bruker’s exclusive PeakForce Tapping as standard
- Large scan field of 100 × 100 × 15 µm3and atomic lattice resolution on inverted microscopes
- 新的基于工作流的用户界面符合人体工程学和易于操作
- New tiling functionality用于大型样品区域的自动映射以及杂交阶段
- Enhanced DirectOverlay 2 modE对于最精确的相关显微镜
- New Vortis 2 controller具有高速低噪声DAC和尖端位置传感器读数技术
- 最高灵活性和升级性具有广泛的模式和配件
アプリケーション
NanoWizard 4 XP BioScience Data Gallery
Bruker’s BioAFMs allow life science and biophysics researchers to further their investigations in the fields of cell mechanics and adhesion, mechanobiology, cell-cell and cell-surface interactions, cell dynamics, and cell morphology. We have collected a gallery of images demonstrating a few of these applications.
仕様
Operating Modes
标准操作模式
Imaging modes
- 现在有峰值攻击
- 接触模式与横向力显微镜（LFM）
- Tapping Mode™ with PhaseImaging™
Force measurements
- Static and dynamic spectroscopy
- Advanced force mapping
Optional Modes
- Fast Scanning option with up to 150 lines/sec
- Fast QI Advanced mode for quantitative data, perfect for soft samples
- Mechanical properties such as adhesion, elasticity, stiffness, deformation
- Conductivity and charge distribution mapping
- Contact Point Imaging (CPI) with zero force
- 结合位点映射的分子识别成像
- Advanced AC modes such as FM and PM with Q-control & Active
获得控制
- 较高的谐波成像
- Kelvin Probe Microscopy and SCM MFM and EFM (see also QI mode)
- Conductive AFM (see also QI mode)
- STM
- Electrical spectroscopy modes
- 高电压的压电显微镜
- Electrochemistry with temperature control and optical microscopy
- NanoLithography
- NanoManipulation
- 纳米吸引力
- 扫描热AFM
- FluidFM® solution from Cytosurge
- ExperimentPlanner for designing a specific measurement workflow
- RampDesigner™ for custom designed clamp and ramp experiments
- 远程实验控制功能
- DirectOverlay 2 for combined AFM and optical microscopy
- Additional XY or Z sample movement stages available with CellHesion®, TAO™ and HybridStage™ module
アクセサリー
市场上最宽的配件范围
Optical systems/accessories, electrochemistry solutions, electrical sample characterization, environmental control options, software modules, temperature control, acoustic and vibration isolation solutions and more. Bruker provides you with the right accessories to control your sample conditions and to perform successful experiments.
ソフトウェア
新的基于工作流的用户界面redefines user-friendliness
The new V7 software interface guides users through the workflow to set up experiments intuitively and makes it simple, even for users with minimal AFM experience, to progress confidently to generating high-quality data. Each stage of the setup and operation works as an optimized desktop that brings all the vital information into focus with a single click.
- On-screen, context-sensitive help
- 对齐和设置的状态反馈
- 有效的基于任务的实验选择
- 快速访问最喜欢和最近使用的实验
- One-click probe calibration
- Instant overview of the key data
- 多用户环境等成像设施的用户管理
ウェビナー
观看最近的生物防护网络研讨会
我们的网络研讨会涵盖了最佳实践，介绍新产品，为棘手的问题提供快速解决方案，并为新应用程序，模式或技术提供想法。