BioSolids CryoProbe

•Å³C和n - 优化的三重共振NMR探针

•增强¹和N的敏感性> 3倍

•一个数量级更快的数据获取，并显着提高了生产率

•Simultaneous irradiation on ¹³C and ¹⁵N under strong proton decoupling

•非常适合对具有低标记水平和小分子的生物固体研究

•Automatic tuning, matching and magic angle adjustment and lift-assisted sample exchange

•样品温度范围：-20 C至+60 C

•MAS速率高达20 kHz，专门设计的3.2毫米转子

•Standard bore design, compatible with SB and WB magnets.

生物固体冷冻探针可以在生理温度下研究挑战性生物系统，而不会改变其样品组成。

由于蛋白质周围的蛋白质的完整性和温度通常对于维持生物学功能至关重要，因此此特征在结构生物学中非常重要。

Diluted samples, such as labelled proteins in their native environment or small molecules in large assemblies, and samples in natural abundance or with low level of labelling, greatly benefit from the significant boost in sensitivity of this innovative probe.

在药理环境中，此探针允许扩展NMR工具箱。2d见C³C相关实验通常用于自然丰度中活性药物成分的高级表征。其他应用领域包括药物多态性和药物递送系统

更快的数据获取

淀粉样蛋白原和prions

〜96.7毫克（u-κC，n）Het-S（218-289）Prion域，由教授提供。A.Loquet, CNRS, Bordeaux, France.

由于光谱的增强敏感性recorded one order of magnitude faster and it’s possible to record the spectra with just a single scan and with an amazing signal-to-noise and resolution.

更快的数据获取
淀粉样蛋白原和prions

〜96.7毫克（u-κC，n）Het-S（218-289）Prion域，由教授提供。A.Loquet，CNRS Bordeaux，法国。

由于光谱的增强敏感性recorded one order of magnitude faster and it’s possible to record the spectra with just a single scan and with an amazing signal-to-noise and resolution.

This results into a large time saving for the setup and acquisition of multidimensional experiments.

获得一个维度
淀粉样蛋白原和prions

〜96.7毫克（U- 13C，15N）HET-S（218-289）Prion域，由教授提供。A.Loquet，CNRS Bordeaux，法国。

通过Biosoldis冷冻探针提供的灵敏度，扫描的数量可以大大减少。结果，多维实验变得更快。然后可以在通常需要运行2D实验的同一时间执行3D实验。另外，4D实验，由于收购时间太长而通常不会进行，因此可以访问，需要少于一周的实验时间。

Explore Low Sensitivity Samples
Large biological assemblies

~ 7.5 mg of U – ¹³C, ¹⁵N – labeled Kif5b, a 349-residue protein, complexed with microtubules. Total sample amount ~ 80 mg, courtesy of prof. T.Pollenova, U.Delaware, USA.

Thanks to the sensitivity enhancement more challenging samples, more complex or less labeled, become the focus of the researcher.

We have recorded 3D NCACX and NCOCX experiments have been recorded on sample constituted of a labelled protein of about 350 residues in a complex with an unlabelled protein (total The total amount of isotopically labeled material is ~9 %).
These experiments, which lasted for ~5 and 6 day respectively, demonstrate the stability of the probe over several days of accumulation under multiple steps of simultaneous irradiation on all RF channels.

生物样品在其本地环境中
Bacteria cell envelope of mycobacteria

~ 40 mg ¹³C, ¹⁵N-labeled cell wall, HEPES 50 mM pH 7.0, courtesy of prof. J.P.Simorre, U.Grenoble, France.

Thanks to the sensitivity enhancement, the researchers can look with solid state NMR directly into cells.

This probe is a game changer for unstable samples that tends to degrade fast over time. On these kind of samples is crucial to be able to collect all the data shortly after they production.

天然产品的天然产品
Wood and Cellulose

Wood from Bordeaux wine threes, courtesy of prof. A.Loquet, CNRS Bordeaux, France.

木材和其他富含纤维素的样品通常呈现无定形和结晶材料的混合物，因此是大小信号的混合物。bob综合游戏区分不同的重叠光谱成分在实验中通常并不容易。有时能够通过二级CC相关实验改善分辨率是分配光谱成分的唯一可行方法。随着生物固体的敏感性的提高，冷冻螺旋桨的重新聚焦不足成为常规工具。

天然产品的天然产品
本地胶原蛋白内部骨基质

山羊皮质股骨（Capra Hircus，2-3岁）。N.Sinha，印度生物医学研究中心。

了解胶原蛋白在健康和患病状况下胶原蛋白在其真正的本地状态下的结构和功能行为是开发各种骨变性疾病，组织工程和骨植入物治疗的里程碑。胶原蛋白的自然丰度光谱通常具有低灵敏度和有限的同位素浓度。
得益于该探针的灵敏度增强，现在可以记录自然同位素丰度中胶原蛋白的高分辨率2d 2d h-¹h-³c。这些光谱在短时间内的接触时间记录下来，阐明了骨基质内部在其真实天然环境中胶原蛋白的短距离相互作用。通过此探测，研究人员在理解骨基质内胶原蛋白的结构稳定性和功能机理方面迈出了一步。

In-situ Characterization of Active Pharmaceutical Ingredients
基于环糊精的金属有机框架（CD-MOF）

由教授提供的CDMOFS颗粒含有〜20％兰索拉唑。C.Martineau-Corcos，Cemhti UPR CNRS 3079，ILV UMR CNRS 8180和法国IUF。

药物传递基质通常是类似于海绵的多孔材料。bob综合游戏金属有机框架（CD-MOF）就是一个很好的例子，因为它们是具有巨大空体积的材料。bob综合游戏加载到MOF中的药物代表了稀释的自旋系统的情况，其中缺乏灵敏度代表了活性成分的原位表征的一个大问题。

It is worth to note that the cryoprobe technology is so far the only tool that allows probing the state of a low API content in these kind of samples. Other approaches, like fast MAS 1H NMR or DNP have been demonstrated to be not suitable or of limited use, because of lack of resolution and unstability of CD-MOFs in the solvents used for DNP sample preparation.

CP和基于双CP的实验

CPMAS冷冻探针是针对结构生物学的标准和高级基于CP的实验开发的。

实验室

Service & Life Cycle Support for Magnetic Resonance and Preclinical Imaging

布鲁克’s commitment to provide customers with unparalleled help throughout the buying cycle, from initial inquiry to evaluation, installation, and the lifetime of the instrument is now characterized by the实验室service concept.

实验室Maintenance Agreements, On-Site On-Demand and Enhance Your Lab are designed to offer a new approach to maintenance and service for the modern laboratory

1。NPM1exhibitsstructuralanddynamicheterogeneityupon阶段separation和theP14arftumorsuppressor
Eric Gibbs, Barbara Perrone, Alia Hassan, Rainer Kümmerle, Richard Kriwacki.
磁共振杂志，2020，310，10646

2。CPMAS的灵敏度提高CryoProbe用于挑战性的生物组装
Alia Hassan, Caitlin M.Quinn, Jochem Struppe, Ivan V.Sergeyev, Chunting Zhang, Changmiao Guo, Brent Runge, Theint Theint, Hanh H.Dao, Christopher P. Jaroniec, Mélanie Berbon, Alons Lends, Birgit Habenstein, Antoine Loquet,Rainer Kuemmerle，Barbara Perrone，Angela Gronenborn，Tatyana Polenova。
Journal of Magnetic Resonance, 2020, 311, 106680

3.Efficient incorporation and protection of lansoprazole in cyclodextrin metal-organic frameworks
Xue Li, Marianna Porcino, Charlotte Martineau Corcos, Tao Guo, Ting Xiong, Weifen Zhu, Gilles Patriarch, Christine Péchoux, Barbara Perrone, Alia Hassan, Rainer Kümmerle, Alexandre Michelet, Anne Zehnacker-Rentien, Jiwen Zhang Ruxandra Gref.
International Journal of Pharmaceutics, 2020, 585, 119442

4.生物固体在骨基质内部的天然胶原蛋白中的短期和远程相互作用。
Nidhi Tiwari, Sebastian Wegner, Alia Hassan, Navneet Dwivedi, RamaNand Rai, and Neeraj Sinha.

在化学中接受的磁共振