MBT Mycobacteria RUO Module

Punti salienti

The genusMycobacterium包括重要的病原体of theMycobacterium tuberculosiscomplex (MTC) and the nontuberculous mycobacteria (NTM). NTM are increasingly the cause of opportunistic infections. This trend and the rise of antibiotic resistance in this genus demand improved differentiation among mycobacterium species.

The mycobacteria identification challenge

The difference in antimicrobial susceptibility among different mycobacteria species makes identification down to the species level crucial for microbiology laboratories. Traditional methods for mycobacteria identification, primarily culture-based phenotypic methods, may take weeks and are often inaccurate. Development of commercially available molecular methods, such as nucleic acid hybridization probes, improved turnaround times significantly, but can lack specificity. Sequencing of target genes like 16S rDNA is very fast and highly accurate, but expensive and not widely available.

Rapid and highly specific identification

晚期质谱（MS）技术的最新出现，特别是基质辅助激光解吸/电离飞行时间（MALDI-TOF）MS，使微生物学实验室能够掌握霉菌杆菌识别的挑战。仪器的无与伦比的速度，例如布鲁克的马尔迪生物基^®(MBT) system, in addition to enhanced ease-of-use, high specificity and significantly reduced cost per test, sees MALDI-TOF MS emerging as a go-to technique for mycobacteria identification.

MBT Mycobacteria RUO Module

The optional MBT Mycobacteria RUO Module for the MALDI Biotyper^®is composed of a specific reference spectrum library and a software module.

The MBT compares the mass spectrum of an unknown organism with the dedicated library of reference mass spectra, which covers 182 of the currently known 201 mycobacteria. This MBT Mycobacteria Library can conveniently be applied for samples independently from growth on liquid or solid medium.

MBT分枝杆菌RUO软件模块允许处理比大多数其他细菌获得的更具挑战性的质谱。标记为分枝杆菌的样本将接受优化的采集方法，并自动将光谱与MBT Mycobacteria RUO库进行比较，以进行敏感和特定的识别。

NEW - MBT Mycobacteria Kit

Mycobacteria are more challenging for MALDI-TOF MS analysis than most other bacteria. Their rigid cell wall requires a harsh method to liberate the ribosomal proteins, which moreover are not so abundantly present due to a typical lower metabolism. As identification by MALDI-TOF MS is mainly based on analysis of ribosomal proteins, a dedicated sample preparation method is needed to render high-quality spectra.
Another essential step of mycobacteria sample preparation is secure inactivation of the bacteria, which should be treated as biosafety level 3 organisms until the identification result is known.

Different to other procedures, the inactivation method of the new MBT Mycobacteria Kit does not involve boiling. The mycobacteria are conveniently inactivated by incubation with an Inactivation Reagent, at room temperature, with mechanical disruption of cell aggregates supporting the inactivation process.
The MBT Mycobacteria Kit is suitable for sample preparation for Mycobacterium spp. cultivated in liquid as well as on solid media and decreases the overall hands-on time from more than 1 hour to less than 45 minutes.