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华盛顿，2019年3月4日 /美通社 / - 15bob体育竞技风暴^th美国人类蛋白质组组织年度会议（www.ushupo.org），布鲁克宣布使用高敏性，高通量质量蛋白质组学的进一步进展timsTOF Promass spectrometer connected to anEvosep Onelow-flow chromatography system. Bruker also highlights advances in峰,Protein Metrics， 和MaxQuant4D proteomics software supporting routine acquisition and information extraction using large-scale accurate collision cross section (CCS) values.

ThetimsTOF Prowith its unique PASEF (Parallel Accumulation Serial Fragmentation) capability allows the acquisition of ion mobility information at high throughput and with ultra-high sensitivity, resulting in the large-scale calculation of accurate collisional cross sections. Using novel 4D algorithms in峰Studio软件导致在192个败血性休克患者中的量化蛋白质急剧增加，以每天100个样品的速度获取：

Dr. Roman Fischer, Nuffield Department of Medicine, Project Leader - Discovery Proteomics, at Oxford University, Oxford, UK, said: "The 4D feature matching capability enabled by TIMS, boosts the number of quantified plasma proteins in a single 11.5 min LC-MS/MS run with low sample amounts injected. Collectively, there were 772 plasma proteins quantified with 100ng injected using these short gradients, where 66% of the identifications correspond to only 10% of the protein mass of plasma. This demonstrates that the method can achieve sufficient depth to discover biomarkers on low abundance proteins. Achieving this depth on thetimsTOF Prooffers completely new possibilities to analyze large sample cohorts of hundreds to thousands of samples for biomarker discovery in blood plasma."

加里Kruppa博士，Bruker Daltonics的副总统蛋白质组学，评论说：“实现更大蛋白质组学深度的一种常见做法是样本前进行分级。但是，这会导致长期测量时间，通常通过多重和化学标记来补偿。尽管同样的标签似乎有吸引力。原则上，它们遭受比率失真，可以偏向或损害样品之间低丰度蛋白的定量。一种无标签的定量方法，将短LC梯度与4D匹配的高选择性相结合，仅在该方法上可用timsTOF Pro，并提供了一种引人注目的替代方案，可以应用于高通量蛋白质组学中的数千个样品。”

美国HUPO 2019中显示的关键新软件开发包括发布峰On-line为了timsTOF Pro, a server software that is parallelized with the ability to run on clusters or multi-CPU machines. In combination with the new denoising algorithm intimsTOF Proacquisition software, which reduces the data file size by a factor 5x to 10x without any noticeable loss of information, the scalable performance of PEAKS Online facilitates state-of-the-art high-throughput proteomics experiments.

Protein Metrics Inc（PMI）还为其Byonic数据库搜索算法添加了肽CCS支持，该算法被HCP和其他生物制剂的药物和生物制药科学家很好地接受。

埃里克·卡尔森（Eric Carlson）博士Protein Metrics Inc.的总裁兼首席执行官表示：“我们很高兴包括对timsTOF Pro和我们的软件中的PASEF数据为客户。对于诸如二硫键键分析，糖划分和宿主细胞蛋白等应用，将离子迁移率和CCS数据包括为4^thdimension of separation, will provide even deeper insights into protein samples to drive drug discovery and development."

Please join Bruker's proteomics team today, March 4^th, at 12:35 - 1:20 pm in the Regency Room for the Bruker Lunch Seminar at US HUPO with the keynote talk: "Unlocking the Power of the Fourth Dimension: MaxQuant for TimsTOF Pro”,博士。Juergen Cox, Research Group Leader at Max Planck Institute of Biochemistry, Martinsried, Germany.

About the timsTOF PRO with PASEF

The proprietarytimsTOF Prosystem uses PASEF, enabled by Trapped Ion Mobility Spectrometry (TIMS), to provide industry-leading data acquisition speed for shotgun proteomics. The unique dual TIMS geometry of thetimsTOF Pro，结合TIMS设备中离子数据包的时间聚焦，这意味着Pasef提供的速度优势随着灵敏度和定量的同时改善。所有这些在速度，灵敏度和定量方面的提高都保持了Bruker高性能QTOF质谱仪的优势，包括高质量分辨率（即使在最高获取速率下的50,000 FWHS解决功率）在MS和MS/MS模式下，PPM精确质量，精确质量，精确质量，精确质量，精确质量。和高的同位素保真度（真正的同位素模式或尖端^TM）。坚固的timsTOF Prowith PASEF gives scientists the tools to dig deeper into the complex cellular machinery with the potential to discover low-level, biologically significant peptides or proteins, or validate them in translational proteomics research.

About Bruker Corporation（纳斯达克：BRKR）

Bruker is enabling scientists to make breakthrough discoveries and develop new applications that improve the quality of human life. Bruker's high-performance scientific instruments and high-value analytical and diagnostic solutions enable scientists to explore life and materials at molecular, cellular and microscopic levels. In close cooperation with our customers, Bruker is enabling innovation, improved productivity and customer success in life science molecular research, in applied and pharma applications, in microscopy and nanoanalysis, and in industrial applications, as well as in cell biology, preclinical imaging, clinical phenomics and proteomics research and clinical microbiology. For more information:www.energie2point0.com.

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