马萨诸塞州比勒里卡（Billerica） - （商业资讯） - 1月。8，2009-布鲁克·达尔顿蒂克（Bruker Daltonics）今天宣布，圣路易斯华盛顿大学的迈克尔·格罗斯（Michael Gross）教授的实验室订购了一个包装，该包装包括Bruker 12 Tesla FTMS和Maxis UHR-TOF，主要用于用于在上层蛋白质组学中申请与在华盛顿大学负责蛋白质组学工作的主管里德·汤森（Reid Townsend）教授密切合作。研究人员还计划将Maxis用于其他重要应用，包括精确的质量公式测定小分子和蛋白质结构研究，这些研究采用其独特的方法称为PLIMSTEX，SIMSTEX和FPOP（OH自由基足迹）。
在自上而下的蛋白质组学中，首先测量完整的蛋白质以确定其分子量，然后在气相中碎片所选蛋白质，并以FTMS的超高分辨率和质量精度测量片段。最新的Bruker Esi-QQ-FTM具有高场12 Tesla磁铁具有200万（2,000,000 FWHM）的质量分辨能力，并且是分析自上而下蛋白质组学中非常复杂的光谱的出色工具。通过将蛋白质序列的片段的预期重量与实验结果相关联，可以明确确定重要蛋白质后翻译后修饰（PTM）（例如磷酸化或糖基化）的位置。关于蛋白质修饰的功能重要信息通常在更传统的自下而上的蛋白质组学中丢失。
的力量英尺分不仅提供了最高的质量resolving power and mass accuracy available on any mass spectrometer for such measurements, but also offers the most versatile suite of tools for fragmenting the proteins. Besides the novel Continuous Accumulation of Selected Ions (CASI(TM)) which allows researchers to enrich selectively the signal from a selected protein or one of its large multiply charged fragments, the Bruker FTMS already includes external collision-cell or internal ICR cell Collision Induced Dissociation (CID), as well as Electron Capture Dissociation (ECD) in the ICR cell. Critical to the top-down analysis process are also software tools that eliminate tedious manual data interpretation, such as the unique Bruker SNAP (TM) algorithm.
Furthermore, an agreement between Bruker and the laboratory of Prof. Gross gives his lab early access to the unique, newly developed front-end Electron Transfer Dissociation (ETD) module for FTMS, a new, sensitive and easy-to-use method for fragmenting peptides and proteins in a fast non-ergodic process that again preserves PTMs. Bruker is the world-leader in ETD on its HCT high-capacity ion traps, and has now made this sensitive ETD capability available also on its ultra-high resolution ESI-FTMS systems as a work-in-progress research capability.
化学，免疫学和医学系教授迈克尔·格罗斯（Michael Gross）博士，美国质谱学会杂志的编辑，评论说：“我们和华盛顿大学医学院的合作者都有许多科学问题，可以最好通过自上而下的蛋白质组学来解决。蛋白质修饰的数量和性质对于理解蛋白质功能至关重要。我们很高兴能在其高视野FTMS上与Bruker Daltonics合作，以获取自上而下的蛋白质组学，并使用独特的组合现有的CID和电池内ECD工具，以及新的前端ETD能力的进一步开发和应用。我们希望获得该工具可以帮助我们使用自上而下的方法来帮助我们解决生物学和医学上重要的问题，这将使我们在自上而下的蛋白质组学应用中处于领导地位。”
新的Bruker超高分辨率飞行时间（UHR-TOF）质谱仪Maxis是当今的第一个也是唯一可用的仪器，可实现以前不兼容的超高质量分辨率和非常快速的UHPLC色谱分离的质谱趋势。在MS和MS/MS模式下，高于1 ppm的卓越质量精度，超高质量分辨能力为40,000至60,000，高达20 Hz，动态范围超过5个数量级，高M/Z功能，允许出色的稳定性1.5MDA高分辨率提取的离子色谱图以及出色的灵敏度即使在耦合到最快的可用色谱分离系统时也可以同时保持，从而为广泛的生命科学应用提供强大的质谱仪。Maxis已被证明是定量蛋白质组学，肽和完整蛋白质分析的一种特殊工具，也可以使用独特的SmartFormula（TM）3D方法来快速的小分子鉴定，用于实现在正面的不敏化分子式读出，共同读出，共同读出的，由英国辉瑞公司开发。
"We are delighted that Professor Gross, who has an outstanding record in the development of mass spectrometry for new applications, has chosen Bruker Daltonics as a partner in developing applications in top-down proteomics," commented Dr. Gary Kruppa, Bruker Daltonics' Vice President for Business Development. "We view our ESI-FTMS with high field magnets as the ultimate platform for top-down proteomics and are pleased to collaborate with Professor Gross in this field. The maXis as a high-throughput, high-performance research tool for applications in proteomics and small molecule research, and the collaboration on the development of front-end ETD, make this a very exciting and important research collaboration for Bruker."
Professor Gross observed further: "The maXis will have many applications in our laboratory. The ultra-high resolution of this instrument will allow us to do experiments we have developed in our lab such as PLIMSTEX, SIMSTEX, and other protein mapping strategies at high speed with isotopic resolution of proteins up to 40 kDa. We are very excited about applying the maXis to this work, but we also see that the maXis will be a superb instrument for the more routine work done in our facility, such as confirmation of small synthetic molecules important in chemistry and metabolomics by using accurate mass MS and MS/MS. We are pleased about bringing new tools such as SmartFormula(TM) 3D to our facility and making them available to our collaborators and clients."
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Gary Kruppa博士，+1 978-663-3660，分机。1151
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Source: Bruker Daltonics