nanoElute^®

系统性能是通过3层保护来维持的：

1. Automatic initialization, where the autosampler and pumps automatically run a preparation protocol before sample injection ensuring performance from the first to the last sample.
2. Built-in scheduling of preventive maintenance jobs based on usage patterns and integrated system expertise where the software will suggest how to optimize continued system operation.
3. 精确的系统诊断，自动检测泄漏和阻塞并在图形用户界面中突出显示它们。

Tailor-made instrument methods are automatically generated using the Instant Expertise™ method editor, based on experimental mode, column selection and analysis time, delivering:

• LC-MS/MS method with intelligent, self-optimizing LC separation for higher productivity and expert-caliber results the first time.
• Optimal experimental conditions are automatically calculated from column type and sample characteristics to suit all proteomics samples
• 色谱分辨率保留了最佳同时ID和定量功能

Gradients can be readily modified but most other parameters are hidden although they can still be edited in “expert” mode.

该系统通过标准布局和新型阀解决方案进行预配置，以确保易于使用和系统稳定性。唯一的阀设计可确保用户可以执行三种不同类型的实验，而无需硬件更改和软件控制的流程路径，用于使用陷阱列，绕过陷阱列或直接输注以快速分析简单样品。

The unique valve design and novel instrument configuration enable:

• 软件控制的流道
• Dynamic inclusion of trap column
• Backflushing the sample for sharper peaks

新的纳米柱和独特的后填充陷阱盒架补充了该乐器，并得到了即时专业知识算法的认可。

Compact and dedicated design有了市场经过验证的自动采样器和大容量，单冲程活塞泵支持更长的梯度和广泛的流量范围，以产生有效的OMICS知识。

现代纳米 - ESI质谱法（MS）分析正在增加前端LC分离的压力，以符合MS的稳健性和可靠性，并要求样品分离的绝对最高质量和可重复性。

To ensure good individual system performance as well as to deliver consistency over multiple systems and across different labs, deeper system integration and novel approaches to quality control as well as intelligent system diagnostics are called for. This in turns requires rigorous protocols and automation for all phases of the experiment, driving new approaches to user interface design and data monitoring, as well as a critical rethink of all flow paths and liquid handling parts of the configuration.

The Bruker nanoElute^®设计具有多年的蛋白质组学知识和纳米-UHPLC专业知识。

基于行业领先的组件，再加上常见问题的创新解决方案，它从安装时刻到多年的OMICS知识生成，提供无故障的操作。

When paired with the ultra high-resolution / accurate mass QqTOF, the Bruker impact II and the market proven CaptiveSpray ionization source, the nanoElute^®system is unbeatable when it comes to:

• Data-dependent and data-independent bottom-up proteomics
• 生物标志物发现和验证的蛋白质成型分析
• 完整的蛋白质表征
• 糖和磷蛋白质组学

The ultra high-resolution, accurate mass QqTOF, the Bruker impact II and the market proven CaptiveSpray ionization source are the perfect companions for the nanoElute^®系统。

纳米电子的复杂性^®’s built-in intelligence and applied chromatographic understanding is hidden behind a user interface that is intuitive and easy to use, but also powerful and flexible to operate.

无故障的OMICS知识产生
纳米电子^®系统即使在两种分离模式下非常短的梯度也提供出色的色谱性能。安装了陷阱列后，重点是速度和稳健性，但是由于新的纳米柱和独特的后填充陷阱盒，色谱性能得到了保持色谱性能。

Without a trap column installed, ultimate separation power is delivered where the trap column is elegantly by-passed by virtue of the novel valve geometry.

唯一的仪器配置使用户可以在同一样品批处理中分析“带有陷阱”和“无陷阱”模式的样品，而无需物理更改硬件设置。

具有长柱和梯度的复杂样品的分离已在有或没有陷阱柱的情况下证明。保留时间和区域的可重复性非常适合所有蛋白质组学应用，包括最苛刻的应用程序。

Method
预订的牛血清白蛋白（Bruker，Billerica，MA）被用作简单蛋白质组学样本（25 fmol/µl）的代表。
根据Beck等人制备HeLa细胞裂解物，并用甲酸酸化为200 ng/µl的最终浓度。对于简单样品，在新型的纳米uhplc系统上使用10分钟的反向相梯度（10分钟2％-35％的溶剂B在10分钟内 - ACN中的2％-35％溶剂B）在新型纳米uhplc系统上分离了胰蛋白酶肽，在ACN中为0.1％FA），在标准化的15cm 75 cm 75 cm的孔柱上，带有带有的标准化。C18树脂。在带有C18树脂的标准化的40厘米75µm孔柱上，用反向相位梯度分离HELA样品（在90分钟内从2％-24％，10分钟内ACN中的24-48分开）。数据是用配备了捕圈的离子源的Image II QTOF MS（Bruker Daltonik，Bruker Daltonik）获得的。

参考
[1] Beck, S., Michalski, A., Raether, O, Lubeck, M., Kaspar, S., Goedecke, N., Baessmann, C., Hornburg, D, Meier, F., Paron, I., Kulak, N.A., Cox, J. and Mann, M. “The Impact II, a Very High-Resolution Quadrupole Time-of-Flight Instrument (QTOF) for Deep Shotgun Proteomics.” Mol Cell Proteomics. 2015 Jul; 14(7): 2014-29.

Direct infusion for fast analysis

独特的阀设计还允许快速运行简单样品的“直接输注”，例如BSA为2μL/min。

Direct infusion for fast analysis
独特的阀设计还允许快速运行简单样品的“直接输注”，例如BSA为2μL/min。