塞拉普尔®-32 Pro
高通量
八个通道/四个斑点的阵列可在24小时内进行13,200个互动
强调
针对复杂的高通量应用程序量身定制
Features
将行业领先的绩效与高通量SPR分析相结合
关键系统具有启用灵活的仪器操作和强大的测定开发，同时保持系统性能，吞吐量和检测灵敏度。
- 32 individually addressable detection spots comprising 8 flow cells with 4 spots to analyze more targets and controls per injection
- 连续流动动力分离微流体能够在溶液之间快速过渡，以在多种样品基质中进行精确动力学
- 单个针头控制可以在每次注射1到8个样品之间进行处理
- 灵活的缓冲式配置节省了时间和样本
- 用户友好的软件提供高通量SPR数据收集和分析
Breakthrough SPR Detection for Sensitivity
SPR+检测器通过将成像表面等离子体共振（SPRI）与高强度激光光源和高速光学扫描相结合而获得其灵敏度。这种布置支持相对较大的二维传感器阵列的极其敏感的成像，而光源的强度允许使用高速摄像头，从而每次扫描都会收集更多的共振测量值。净结果是测量小响应变化时的信噪比为0.02 RU（RMS），并提高了准确性，这对于片段或小分子结合实验经常观察到。
Automated high-throughput sample analysis
With 32 detection spots available during each analysis cycle, several user-defined configurations are possible to maximize high-throughput SPR analysis. This can include 31 control-subtracted injections, up to 3 in-line controls or 4 targets per channel. The autosampler is compatible with a wide range of 96- and 384-well microtiter plates and the dual-plate sample deck with common rack position allows for increased automation.
When equipped with an optional plate handling robot, the instrument can function in high-throughput mode and can analyze more than 4400 samples per day, resulting in more than 13,200 control-subtracted interactions.
塞拉普尔®-32 Pro can be easily integrated into an automated scheduling software via API.
框架注入功能用于多缓冲区分析
八个注射器连续流缓冲区是一个强大的测定开发和优化工具。它还支持在一系列条件下（包括不同的pH，盐，洗涤剂或溶剂浓度）的高通量SPR分析。同时进行多支无用的分析节省了时间和试剂，同时增加了信息吞吐量。
好处
高通量with performance
Sierra Spr®-32 Pro system is a robust instrument designed for high-throughput applications such as epitope characterization and antibody, fragment or small molecule screening.
Simultaneous injection of up to eight samples facilitates high-throughput assay development and optimization, as well as rapid quantitative analysis of both crude and purified samples. Hydrodynamic isolation (HI) continuous flow microfluidic technology addresses samples as flowing streams onto the sensor array. This allows processing of 8 samples on a standalone system, which can be boosted to more than 4400 samples per day through optional plate handling robotics.
灵活性优化生产率
Individual needle control (INC) optimizes the performance, flexibility and scope of applications, while saving time and materials when throughput is not the main requirement.
最多可以使用框架注射在仪器中同时使用八个缓冲区。在单个测定中评估不同pH值，离子强度，清洁剂或溶剂浓度以及副因素或抑制剂的效果的能力会导致更简单，优化的测定最终提高吞吐量和生产率。每个通道设计的四传感器设计为控制分析提供了最大的灵活性，同时为最复杂的分析保持吞吐量。
Assay control and sensitivity
在塞拉普尔SPR的八个通道中的每个通道中的每个通道中，都配置了四个独立寻址的检测点®-32 Pro系统。可同时测试样品ed on up to three control surfaces as well as the active surface. In many SPR applications, early detection of nonspecific binding to control proteins is critical.
In small molecule screening and characterization, binding to serum proteins such as HSA and BSA is an integral part of drug development. The additional in-channel control halves the number of assay cycles required and improves data quality by eliminating potential variations between multiple sample preparations.
The SPR+detector combines imaging SPR (SPRi) with a high intensity laser light source and high-speed optical scanning. The result is improved signal-to-noise and improves accuracy when measuring small response changes.
Ease of use
From initial assay development to pre-defined method templates, the Sierra SPR control software provides the perfect solution for every user. It combines the flexibility of the hardware with an easy-to-use drag and drop method editor. A well defined plate and method library supports personal workflow structuring and the data view allows real-time visual monitoring of flow cells.
消耗品
更多信息
表面等离子体共振的应用
通过在诺华的高通量生物物理筛查来解决具有挑战性的目标
Learn in this note how multiplexing, automation and automated data analysis with Genedata Screener take SPR to the next level in this real-life example.
如何使用胺耦合影响配体密度
找到理想的固定水平以获得最佳的动力学测量至关重要。在这里，我们检查了不同示例目标上的影响参数。
Screening and characterization of small molecule binding to protein targets
通过SPR检测的实时，无标签（RT-LF）分析是小分子药物和候选药物的生物物理表征的强大工具。
筛选和表征生物治疗学
通过SPR检测的实时无标签（RT-LF）分析是蛋白质疗法生物物理表征的强大工具。
使用框架注射对条件依赖性结合的研究
The traditional investigation of condition-dependent binding demands high reagent costs as well as lengthy run time. Frame inject delivers cost and time savings at low sample consumption.
文学室表面等离子体共振
支持
联系专家
仅用于研究。不用于临床诊断程序。