NanoTracker 2

使用Nanotracker 2，用户可以捕获和跟踪粒子从几个µm降低到30nm，并能够以纳米精度和femtonewton分辨率实时控制，操纵和观察样品。

NanoTracker technology provides precisely quantifiable and reproducible measurements of particle/cell interactions. The system delivers precise information about single molecule mechanics and may also be used to determine mechanical characteristics such as adhesion, elasticity or stiffness on single molecules.

The video shows confocal scanning microscopy combined with optical particle manipulation

The new system is designed to detect the smallest forces and manipulate particles or molecules with the highest precision. Special laser stabilization and newly designed detection electronics in the head provide to very low noise levels. Additionally, the compact folded design of the laser beam path makes the system immune to drift.

Double-beam or multi-beam configurations, combined solutions for coarse and extra precise sample positioning give the user flexibility. Several beam steering options including the newly designed pivot-point piezo-driven mirrors and fast acousto-optic deflectors (AODs) perfectly match requirements of any application.

除了广泛的样本定位控制器l including a customized closed-loop piezo sample stage option, the traps can be steered individually in 3D through the sample. Moreover, the laser power can be controlled for both traps independently. This freedom is required to allow a wide range of experimental assays and geometries.

The two traps are available full time and are generated from a single laser source by polarization splitting. This makes the system ultra-stable against drift.

The new back focal plane interferometry detection unit of the NanoTracker 2 is equipped with individual detectors for each trap having separate diodes to lateral (XY) and axial (Z) displacements of the trapped bead.

这种检测方法结合软件控制的调光滤波器，允许使用全部动态范围，从而实现了对任何选定的珠类型，激光强度和陷阱拆分比的最高敏感性。

是重要的力量surements are precise calibration of the traps, lowest position noise and a flat trap stiffness profile over a large field of view. The new precise and flexible one-button trap calibration procedure is independent from bead size and medium viscosity. The cross-talk between trap signals in the detection is drastically reduced.

• Intra-molecular elasticity & protein folding dynamics
• Motor protein tracking
• DNA/RNA mechanics
• Protein-DNA binding
• Nanopores & 3D polymer network probing

• Membrane organization (e.g., lipid rafts)
• Trans-membrane processes, trafficking
• 细胞内力
• Receptor-ligand experiments
• Cell mechanics and cell motility
• 膜系绳动力学
• Micro-rheology of cells and gels

• Tracking of pathogen-host interaction and escape forces
• Bacterial and virus adhesion forces
• 局部基因或药物输送
• Entrance mechanism studies
• 纳米毒性和内吞作用研究

• 复杂的光学陷阱几何形状
• Optical guiding & artificial crystal building
• Local field enhancement & Raman/SERS applications
• Brownian motion tracking, Photonic Force Microscopy (PFM)
• Colloidal and polymer meshworks force probing
• Video particle tracking and optical spectroscopy

• 3D force measurements with femto-Newton sensitivity and sub-nm precision
• 最高稳定性和最低的噪声水平以最准确的测量值
• Simultaneous fluorescence imaging
• Powerful, flexible control and data analysis software
• Class 1 laser certified
• 从单分子到活细胞的柔性，模块化设计

力量的BioAFMs允许生命科学和生物物理学researchers to further their investigations in the fields of cell mechanics and adhesion, mechanobiology, cell-cell and cell-surface interactions, cell dynamics, and cell morphology. We have collected a gallery of images demonstrating a few of these applications.

Instrument modes

• 3D粒子跟踪
• Point and Trap for easy trap positioning
• Online calibration based on power spectrum
• 带有新的JPK RampDesigner的高级力量光谱包括力夹和力坡道
• Active and passive force mapping
• Micro-rheology mode
• Optical sorting
• 陷阱振荡
• Multiplexing for 3D trap arrays
• Line traps & circular traps
• Microfluidics control
• 纳米组装
• 光学Z堆叠

Advanced optical microscopy modes

• Brightfield transmission illumination (standard)
• Differential Interference Contrast (DIC) in transmission (standard)
• Epi-fluorescence microscopy (standard)
• Raman spectroscopy
• TIRFmicroscopy
• 共聚焦显微镜
• FRET microscopy and more

Optical systems/accessories, electrochemistry solutions, electrical sample characterization, environmental control options, software modules, temperature control, acoustic and vibration isolation solutions and more. Bruker provides you with the right accessories to control your sample conditions and to perform successful experiments.