MBTStar®-CEPHA IVD测定法
Highlights
MBTStar®-CEPHA IVD测定法
To counteract the rise of multidrug-resistant (MDR) bacteria, Bruker is dedicated to developing new solutions to keep pace. The rapid identification and determination of resistance is vital for clinicians and physicians to provide their patients with the antibiotic that promises rapid, gentle and appropriate treatment, as quickly as possible.
Countering multi-drug resistance bacteria
对3的电阻率rd头孢菌素的一代 - 治疗革兰氏阴性细菌的重要药物 - 近年来一直在稳步上升，并且对全球公共卫生（尤其是医疗保健环境）非常关注。头孢菌素耐药性为世界上某些病原性感染留下了有限的治疗选择，这迫切需要快速，准确的耐药性检测方法以及新的抗生素的发展。
The MBT STAR®-Cepha IVD分析检测到3rdgeneration cephalosporins within just one hour after a positive blood culture alert, by analyzing the activity of cephalosporinase enzymes produced by the bacteria. Newer technologies, such as Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight mass spectrometry (MALDI-TOF MS), are now being used for specialized assays such as the MBT STAR®-Cepha IVD Assay to combat the MDR bacteria threat.
Features
Detecting cephalosporinase activity
Together with Bruker’s IVD-CE MALDI Biotyper®(MBT), the MBT STAR®-Cepha IVD Kit enables the detection of the characteristic enzymatic hydrolysis of the β-lactam ring of 3rd generation cephalosporin antibiotics. The level of hydrolysis is measured by acquisition of mass spectra in the typical mass range of the antibiotic. The assay allows detection of most actively expressed extended-spectrum beta-lactamases (ESBL) and AmpC β-lactamases in MDR gram-negative Enterobacterales:
ESBL:
- e.g. plasmidic TEM, SHV and CTX-M
AmpC:
- Chromosomal and plasmidic
- Inducible or overexpressed resistance genes
- e.g. AmpC, FOX, LAT, DHA and CMY.
Simple workflow, fast results
Starting from a positively flagged blood culture, processed by the rapid MBT Sepsityper®workflow, or a (sub)culture plate, results are typically available within 60 minutes.
For this purpose, bacteria are incubated with a benchmark 3rd generation cephalosporin antibiotic, conveniently provided in a microtiter plate contained in the kit.
测定孵育时间为30分钟后，将等分试样转移到MALDI目标板上，并被专用MBT星覆盖®Matrix, and analyzed at the level of the antibiotic, using the IVD-CE MALDI Biotyper®.
The presence of cephalosporinase activity results in specific mass shifts of the antibiotic, evaluated by the dedicated MBT STAR®提单试管软件模块or MBT Compass IVD. Acquired mass spectrometric data are processed and the ratio of hydrolyzed to non-hydrolyzed cephalosporin antibiotic is calculated automatically and instantly in the background by the MBT STAR®-bl IVD模块。用户收到最终报告，包括测定结果解释，通过简单的颜色编码表示酶活性的状态，从而促进。
Please contact your local representative for availability in your country.
Not for sale in the USA.
More Information
More information
As of May 2021, Bruker Daltonik GmbH is now Bruker Daltonics GmbH & Co. KG.
