Direct Imaging in tissue sections

MALDI Imaging

MALDI Imaging is a powerful mass spectrometry tool for mapping the distribution of molecules from a thin sample, ranging from small metabolites to large proteins, without molecular tags or labels.

MALDI成像 - 揭示了更大的分子见解

单个MALDI成像测量可产生多达数千个分布图或离子图像,以揭示对分子构成和区域异质性的更深入的洞察力和理解。

- Label-free mapping of many analytes, metabolites and proteins

- 图像数百至数千种化合物

- Probe the molecular heterogeneity of samples

- 确定区域特定的分子差异

- Seamless integration with microscopy or histology

分子成像重新定义

Whether your research is focused on植物代谢组学,细胞过程的生物化学,疾病开发或治疗的生物标志物,或者microbial interactions,Maldi Imaging可以为您的工作提供新的见解。这样做的关键是能够将MALDI成像与您可能已经使用的其他成像方式集成,例如荧光显微镜或组织学。

布鲁克(Bruker)在2005年启用了这一能力,该功能允许将其他图像的导入和注册到MALDI成像数据集中,并具有完整的视觉覆盖。

Four proteins distributed in rat brain. Two insets show protein ion Images overlaid with histology image. Note the strong localization to sub-structures.

MALDI成像的应用区域多样性和增长,这是由于该技术的无标签性质以及通过分子量区分化合物的能力。诸如临床研究中的未靶向发现研究试图发现新颖的疾病和治疗生物标志物。有关智能的最大信息,请使用MALDI引导的SpatialOMX®。MALDI成像还通过提供治疗化合物及其代谢物的直接分配监测来彻底改变临床前药物发现管道。

这se are two examples ofuntargeted或者有针对性的方法where MALDI Imaging has proven valuable. Newer applications surrounding plants, polymers and microbes can be seen emerging in the scientific literature where Bruker's MALDI Imaging Technologies have proven valuable.

MALDI Imaging Technologies

Using MALDI to spatially measure molecular signatures
25多年来,布鲁克一直是MALDI质谱创新的领导者。MALDI适用于任何分析任务,这在科学文献中得到了例证,其中MALDI成像出版物在下一个电离技术中占据了5:1的主导地位。Bruker提供了MALDI成像系统,这些成像系统结合了新型MALDI技术,以完成任何有针对性或未定位的成像研究。

Spatially oriented molecular signatures
Images are generated by collecting MALDI data in a virtual grid pattern applied to a sample surface. Thin sections of samples are mounted onto conductive glass slides and a layer of MALDI matrix is applied by aerosol. Bruker’s MALDI Imaging technologies allow the user to define the measurement area and resolution directly using a digital image of the sample. The size of the virtual grid establishes the image resolution and is tailored for the experimental question to be answered.

鉴于每个MALDI频谱都是从离散的虚拟网格中获取的,而无需过采样,因此结果数据集是采样独特区域的分子标志。与使用过度采样的电离系统不同,布鲁克的MALDI成像技术产生的离子图像不会显示出“模糊”图像。

Imaging Technologies - Analytical Versatility and Imaging Speed

对于生物标志物发现成像研究,Bruker的Flex系列MALDI-TOF和TOF/TOF系统提供了最大的分析多功能性和成像速度。因此,MALDI-TOF平台是科学文献中最广泛使用的成像系统。

Highest Molecular Specificity

Untargeted and targeted studies of smaller molecules demand high analytical performance for separating isobars and/or near-isobars. Bruker’s MRMS and timsTOF fleX systems meet the challenge by offering the highest molecular specificity available.

For targeted and untargeted imaging studies, Bruker’ssolariXScimax®Magnetic Resonance Mass Spectrometers(MRMS)提供极端的质量分辨率。在成像速度下,达到> 290,000 mass resolution at m/z 400capable of differentiating and mapping compounds that differ by only a few mDa.

timsTOF fleXSpatialOMX®所需的多功能性。建立在布鲁克的开创性timsTOF Proplatform with PASEF, the timsTOF fleX powers all your X-Omics analyses, while adding a high spatial resolution MALDI Imaging source to deliver a spatial dimension to OMICS analyses. Transform proteomics analysis into spatial proteomics, lipidomics into spatial lipidomics, and metabolomics into spatial metabolomics - SpatialOMx®.

scilsautopilot: Automated workflows to simplify measurement setups

我们的软件解决方案使得从MALDI成像开始变得容易。这scilsautopilote.g., is a new software workflow for simple and reproducible setup of MALDI Imaging measurements relying on Bruker’sIntelliSlides™。自动化步骤节省了时间并减少手动用户输入,可提高测量的可靠性和可重复性,无论用户的专业知识如何。

自动化工作流程可用于flex的timstof,timsTOF fleX MALDI-2和Rapiflex。Please download a Quickstart guide here for theTimstof Flex,包括MALDI-2Rapiflex

Related Content

Biomarker discovery

Molecular changes originate at the cellular level and solution-based techniques lack sensitivity to capture changes produced from small cell populations. MALDI Imaging of sample cohorts along with SCiLS™ Lab analytical software extracts unique spatial patterns of proteins, lipids or metabolites and correlates them with sample pathology to identify and map putative biomarker ions.

Targeted imaging

Choosing between quantitation, distribution or molecular specificity? Imaging offers many advantages for targeted imaging in the drug discovery pipeline. It offers a unique combination of molecular specificity and distribution mapping that neither Quantitative Whole-Body Autoradiography (QWBA) nor LC-MS/MS can deliver.

海报大厅马尔迪成像

Bruker’s MALDI Imaging systems are impacting a wide range of research fields. Inside this poster hall we present a selection of recent scientific presentations that employ MALDI Imaging or SpatialOMx® to answer a variety of biological questions. Please browse the posters to see how the versatility of MALDI Imaging may help you overcome research challenges.

谱技术指导SpatialOMx®

MALDI引导的无靶向发现的SpatialOMX®提供了最完整的分子范围,并提供了上下文化的空间信息,为癌症生长不可或缺的细胞间通信网络提供了重要的线索。

For Research Use Only. Not for use in clinical diagnostic procedures.