Vutara VXL

• SMLM具有高Z分辨率用于风暴，手掌，油漆和相关的超分辨率应用
• 具有大型FOV的定位显微镜，用于超分辨率多路复用基因组应用的优化

• Epi-fluorescence microscopy with large FOV
• Transmitted light microscopy with large FOV

• 405 nm, 120 mW

• 488 nm, 2000 mW (optional)

• 555 nm, 2000 mW

• 638 nm, 1200 mW

• 750 nm, 1500 mW (optional)
  

• Flat excitation profile guaranteed by top hat illumination from a square fiber
  

• Up to 5 colors sequential

• Simultaneous imaging of two focal planes allows 1 µm depth discrimination (larger z range possible in z-stack mode)

Camera

• Orca Flash 4.0 V3 sCMOS camera
• Orca Fusion BT sCMOS camera (optional)

• 60x magnification
• 1.3 numerical aperture (NA)
• Silicon oil immersion
• 0.3 mm working distance
• #1.5H cover glass (0.170 ±0.005 mm)

• 200 µm x 200 µm for multiplexed localization microscopy and widefield imaging
• 使用开关（风暴，棕榈，油漆）和3D定位（双翼飞机检测）的SMLM 50 µm x 50 µm
• Larger FOV with tile scanning

• Fitting of measured PSF to maximize accuracy in xyz

• Proprietary biplane for high accuracy z-resolution and deep sample imaging
  

• 20 nm laterally (xy)
• 50 nm axially (z) with biplane

• > 30 µm (typical, dependent on sample)
  

• SMLM具有高Z分辨率用于风暴，手掌，油漆和相关的超分辨率应用
• 具有大型FOV的定位显微镜，用于超分辨率多路复用基因组应用的优化

• 有限公司urse focus to localize sample
• Fine focus for fast z-stack acquisition

• 数据获取期间的主动焦点漂移校正
• Focus drift <30 nm over 10 minutes
• xy drift correction post-acquisition

• Live cell incubation with humidity, CO2, and temperature control (optional)
  

• Multiplexed exchange of sample buffer (optional fluidics unit)

• 15 x 2 ml reservoirs for imaging probes

• 2 x 15 ml, 1 x 50 ml, 1 x 100 ml for washing and buffer solutions

• 1 µL/min – 1000 µL/min flow rate

• Seamless integrations in SRX microscope control software
  

• Space required if microscope and laser launch are installed on same table: 120 cm x 75 cm (4 ft x 2.5 ft)

• Microscope without camera:

34 cm x 40 cm x 34 cm, (1.2 ft x 1.3 ft x 1.2 ft)

35 kg (78 lbs)

• Laser launch:

33 cm x 53 cm x 32 cm (1.0 ft x 1.7 ft x 1.0 ft)

30 kg (65 lbs)

• Electronics box stacked on top of laser launch:

53 cm x 35 cm x 14 cm (1.7 ft x 1.1 ft x 0.4 ft)

8公斤（17磅）

• The light-tight design does not require dimmed room light

• The system includes vibration insulation (sturdy table or bench required for installation)

• Optional: Customers can supply optical table for challenging environments
  

• SMLM modalities

STORM / dSTORM

PALM

PAINT

Other blinking-based modalities

• Genomics modalities

Chromatin tracing

smFISH (in development)

• Multiplexing workflow

Tracking of probe cycles and positions

Support for an unlimited number of probes

• Calibration workflow to ensure superior localization precision
  

• xy fitting based on measured point-spread function (PSF)
• z位置determined from calibrated biplane data
• Initial localization during data acquisition
• 验证本地化的统计工具

• Diffusion analysis based on nearest neighbors

• Particle assignment based on sparse emitter tracking

• Mean-square displacement and distribution analysis and plots

• Mean velocity and angle calculations and plots

• 设置和控制多路复用成像工作流程
• Tracking of probe labels and metadata

• Optical reconstruction of chromatin architecture

Drift correction

• Auto-correlation
• Fiducials embedded in the sample

• Spatial distribution

Ripley’s K functions

Pair correlation

Nearest neighbors

• Cluster analysis

DBSCAN

OPTICS

Delaunay

Particle counts

Volume and density calculations

Determination of radius of gyration and sphericity

Principal component analysis (PCA)

质心的计算

• 有限公司localization

Pearson’s correlation coefficient

Mander’s overlap coefficient

Intensity correlation quotient

Cross pair correlation

Joint histogram

STORM-RLA

• Resolution analysis

Fourier ring correlation

Labeling density resolution analysis

Local resolution correlation mapping

最近的邻居分析

• 2D和3D可视化
• Point cloud and point splatting
• Volumetric slices
• 等法表
• Maximum intensity rendering
• Depth color coding
• Time frame color coding
• 有限公司lor coding by probe
• Cluster hull visualization
• Wireframe visualization of clusters
• Trajectories for tracked particles

• Raw images are stored in highly efficient binary format and can be exported in ome-tiff format

• Export of drift corrected data

• Localizations are stored in highly efficient binary format and can be exported as .csv or MATLAB binary files

• Results from cluster analysis are stored and can be exported

• Export of results from statistical analysis as .csv or MATLAB binary files

• All imaging and processing settings are stored in xml or Jason format

• Images can be exported as vector graphics or user-defined size/DPI

• Specifications for all data formats are published
  

• 90 TB effective storage

• 2 Gb/sec transfer rate